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Gene Symbol | ALK | ||||||||||
Synonyms | ALK1 | CD246 | NBLST3 | ||||||||||
Gene Description | ALK, anaplastic lymphoma kinase, is a receptor in the insulin receptor superfamily and is a key regulator of neuronal development (PMID: 21502284) and also promotes cell proliferation through activation of MAPK and PI3K signaling pathways (PMID: 27573755). Alk activating mutations, rearrangements, and fusions have been identified in various cancers (PMID: 22649787), including EML4-ALK in non-small cell lung cancer (PMID: 30108712, PMID: 30194140), and a number of mutations confer resistance in the context of Alk fusions (PMID: 25749034, PMID: 21948233). | ||||||||||
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Variant | Impact | Protein Effect | Variant Description | Associated with drug Resistance |
---|---|---|---|---|
A1015T | missense | unknown | ALK A1015T lies within the EGF-like region of the Alk protein (UniProt.org). A1015T has been identified in the scientific literature (PMID: 37000961), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Apr 2024). | |
A1047T | missense | unknown | ALK A1047T lies within the transmembrane domain of the Alk protein (UniProt.org). A1047T has been identified in sequencing studies (PMID: 28524162), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Jun 2024). | |
A1200V | missense | no effect - predicted | ALK A1200V lies within the protein kinase domain of the Alk protein (UniProt.org). A1200V demonstrates kinase activity comparable to wild-type Alk in culture (PMID: 25517749), and therefore, is predicted to have no effect on Alk protein function. | |
A1200_G1201delinsW | indel | unknown | ALK A1200_G1201delinsW results in deletion of an alanine (A) and a glycine (G) from aa 1200 to aa 1201 in the protein kinase domain of the Alk protein, combined with the insertion of a tryptophan (W) at the same site (UniProt.org). A1200_G1201delinsW has been associated with secondary drug resistance to some ALK inhibitors (PMID: 34548910), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | Y |
A1251T | missense | loss of function - predicted | ALK A1251T lies within the protein kinase domain of the Alk protein (UniProt.org). A1251T results in loss of kinase activity in an in vitro assay and is not transforming in cultured cells (PMID: 33674381), and therefore, is predicted to lead to a loss of Alk protein function. | |
A1252V | missense | no effect - predicted | ALK A1252V lies within the protein kinase domain of the Alk protein (UniProt.org). A1252V does not result in Alk autophosphorylation and is not transforming in culture (PMID: 22086496), and therefore, is predicted to have no effect on Alk protein function. | |
A1266D | missense | unknown | ALK A1266D lies within the protein kinase domain of the Alk protein (UniProt.org). A1266D has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, Jun 2024). | |
A1266V | missense | no effect - predicted | ALK A1266V lies within the protein kinase domain of the Alk protein (UniProt.org). A1266V has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function. | |
A195V | missense | no effect - predicted | ALK A195V lies within the extracellular domain of the Alk protein (UniProt.org). A195V has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function. | |
A348D | missense | gain of function | ALK A348D lies within MAM domain 1 of the Alk protein (UniProt.org). A384D confers a gain of function to the Alk protein as demonstrated by increased cell proliferation in the absence of growth factor and colony formation in culture (PMID: 26032424). | |
A528T | missense | unknown | ALK A528T lies within MAM domain 2 of the Alk protein (UniProt.org). A528T has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
A585T | missense | no effect - predicted | ALK A585T lies within MAM domain 2 of the Alk protein (UniProt.org). A585T has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function. | |
act mut | unknown | gain of function | ALK act mut indicates that this variant results in a gain of function in the Alk protein. However, the specific amino acid change has not been identified. | |
amp | none | no effect | ALK amplification indicates an increased number of copies of the ALK gene. However, the mechanism causing the increase is unspecified. | |
C1021V | missense | no effect - predicted | ALK C1021V lies within the extracellular domain of the Alk protein (UniProt.org). C1021V does not result in Alk autophosphorylation and is not transforming in culture (PMID: 22086496), and therefore, is predicted to have no effect on Alk protein function. | |
C1097A | missense | no effect - predicted | ALK C1097A lies within the cytoplasmic domain of the Alk protein (UniProt.org). C1097A is not activating in an in vitro assay and is weakly transforming in culture in one study (PMID: 33674381) but in another study is not transforming in culture, does not lead to ligand-independent kinase activity, and retains functional kinase activity upon ligand binding in culture (PMID: 29084134), and therefore, is predicted to have no effect on Alk protein function. | |
C1156F | missense | unknown | ALK C1156F lies within the protein kinase domain of the Alk protein (UniProt.org). C1156F has been demonstrated to occur as a secondary drug resistance mutation in the context of NPM1-ALK (PMID: 25749034, PMID: 30322862) and other Alk rearrangements (PMID: 36203454), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Jun 2024). | Y |
C1156Y | missense | unknown | ALK C1156Y lies within the protein kinase domain of the Alk protein (UniProt.org). C1156Y has been demonstrated to occur as a secondary drug resistance mutation in the context of ALK fusions (PMID: 21613408, PMID: 20979473, PMID: 27490033, PMID: 27045755), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | Y |
C1235R | missense | unknown | ALK C1235R lies within the protein kinase domain of the Alk protein (UniProt.org). C1235R has been demonstrated to occur as a secondary drug resistance mutation in the context of ALK compound mutations (PMID: 38448512), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Mar 2024). | Y |
C1237Y | missense | gain of function - predicted | ALK C1237Y lies within the protein kinase domain of the Alk protein (UniProt.org). C1237Y results in both increased phosphorylation of Alk and proliferation, and has been demonstrated to confer resistance to Alk inhibitors in the context of EML4-ALK in culture (PMID: 38448512), and therefore, is predicted to lead to a gain of Alk protein function. | Y |
C928Lfs*20 | frameshift | loss of function - predicted | ALK C928Lfs*20 indicates a shift in the reading frame starting at amino acid 928 and terminating 20 residues downstream causing a premature truncation of the 1620 amino acid Alk protein (UniProt.org). Due to the loss of the protein kinase domain (UniProt.org), C928Lfs*20 is predicted to lead to a loss of Alk protein function. | |
C990R | missense | unknown | ALK C990R lies within the extracellular domain of the Alk protein (UniProt.org). C990R results in increased cell proliferation and cell viability compared to wild-type Alk in one of two cell lines in culture (PMID: 29533785), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
D1091N | missense | unknown | ALK D1091N lies within the cytoplasmic domain of the Alk protein (UniProt.org). D1091N results in increased ERK activation in the presence of agonist antibodies (PMID: 23104988), but does not transform cultured cells (PMID: 23104988), and therefore, its effect on Alk protein function is unknown. | |
D1163N | missense | gain of function - predicted | ALK D1163N lies within the protein kinase domain of the Alk protein (UniProt.org). D1163N is not transforming in cultured cells but is activating in an in vitro assay (PMID: 33674381), and therefore, is predicted to lead to a gain of Alk protein function. | |
D1203fs | frameshift | loss of function - predicted | ALK D1203fs results in a change in the amino acid sequence of the Alk protein beginning at aa 1203 of 1620, likely resulting in premature truncation of the functional protein (UniProt.org). Due to the loss of the ATP binding site (UniProt.org), D1203fs is predicted to lead to a loss of Alk protein function. | |
D1203N | missense | unknown | ALK D1203N lies within the protein kinase domain of the Alk protein (UniProt.org). D1203N has been demonstrated to occur as a secondary drug resistance mutation in the context of ALK fusions (PMID: 35123209, PMID: 27432227, PMID: 28434515), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | Y |
D1225N | missense | unknown | ALK D1225N lies within the protein kinase domain of the Alk protein (UniProt.org). D1225N has been identified in the scientific literature (PMID: 37529699), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Apr 2024). | |
D1249fs | frameshift | loss of function - predicted | ALK D1249fs results in a change in the amino acid sequence of the Alk protein beginning at aa 1249 of 1620, likely resulting in premature truncation of the functional protein (UniProt.org). Due to the loss of the ATP binding site (UniProt.org), D1249fs is predicted to lead to a loss of Alk protein function. | |
D1270G | missense | loss of function - predicted | ALK D1270G lies within the protein kinase domain of the Alk protein (UniProt.org). D1270G results in loss of kinase activity in in vitro assays and is not transforming in culture (PMID: 33674381, PMID: 25517749), and therefore, is predicted to lead to a loss of Alk protein function. | |
D1276_R1279delinsE | indel | gain of function | ALK D1276_R1279delinsE results in a deletion of four amino acids in the protein kinase domain of the Alk protein from amino acids 1276 to 1279, combined with the insertion of a glutamic acid (E) at the same site (UniProt.org). D1276_R1279delinsE results in constitutive activation of Alk and is transforming in cell culture (PMID: 37147298). | |
D1311N | missense | no effect - predicted | ALK D1311N lies within the protein kinase domain of the Alk protein (UniProt.org). D1311N does not result in Alk autophosphorylation and is not transforming in culture (PMID: 22086496), and therefore, is predicted to have no effect on Alk protein function. | |
D1349H | missense | unknown | ALK D1349H lies within the protein kinase domain of the Alk protein (UniProt.org). D1349H is not activating in an in vitro assay and is not transforming in culture (PMID: 33674381, PMID: 25517749), but has not been fully biochemically characterized and therefore, its effect on Alk protein function is unknown. | |
D1529E | missense | no effect - predicted | ALK D1529E lies within the cytoplasmic domain of the Alk protein (UniProt.org). D1529E has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function. | |
D1529G | missense | unknown | ALK D1529G lies within the cytoplasmic domain of the Alk protein (UniProt.org). D1529G has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
D94N | missense | unknown | ALK D94N lies within the extracellular domain of the Alk protein (UniProt.org). D94N has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
del exon2 | deletion | unknown | ALK del exon2 indicates a deletion of exon 2 of the ALK gene. | |
del exon2-17 | deletion | unknown | ALK del exon2-17 indicates the deletion of exons 2-17 of the ALK gene. | |
del exon2-19 | deletion | unknown | ALK del exon2-19 indicates the deletion of exons 2-19 of the ALK gene. | |
del exon2-3 | deletion | unknown | ALK del exon2-3 indicates the deletion of exons 2-3 of the ALK gene. | |
del exon3 | deletion | unknown | ALK del exon3 indicates a deletion of exon 3 of the ALK gene. | |
del exon4-11 | deletion | gain of function | ALK del exon4-11 indicates the deletion of exons 4-11 of the ALK gene (PMID: 23139213). Del exon4-11 results in downstream signaling similar to wild-type protein in cultured cells but confers a gain of function to Alk as demonstrated by increased kinase activity in an in vitro assay, increased proliferation and anchorage-independent growth in cultured cells and tumor formation in a mouse model (PMID: 23139213). | |
E1129V | missense | unknown | ALK E1129V lies within the protein kinase domain of the Alk protein (UniProt.org). E1129V has been identified in the scientific literature (PMID: 34890832, PMID: 36093526, PMID: 33161228), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Apr 2024). | |
E1154K | missense | unknown | ALK E1154K lies within the protein kinase domain of the Alk protein (UniProt.org). E1154K has been identified in the scientific literature (PMID: 31585938, PMID: 38448512), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Apr 2024). | |
E1161A | missense | unknown | ALK E1161A lies within the protein kinase domain of the Alk protein (UniProt.org). E1161A is not activating in an in vitro assay and is weakly transforming in culture (PMID: 33674381), but has not been fully biochemically characterized and therefore, its effect on Alk protein function is unknown. | |
E1210K | missense | unknown | ALK E1210K lies within the protein kinase domain of the Alk protein (UniProt.org). E1210K has been demonstrated to occur as a secondary drug resistance mutation in the context of ALK fusions (PMID: 29650534, PMID: 35123209, PMID: 27432227), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | Y |
E1242K | missense | unknown | ALK E1242K lies within the protein kinase domain of the Alk protein (UniProt.org). E1242K is not activating in an in vitro assay and is not transforming in culture (PMID: 33674381), but has not been fully biochemically characterized and therefore, its effect on Alk protein function is unknown. | |
E1303K | missense | unknown | ALK E1303K lies within the protein kinase domain of the Alk protein (UniProt.org). E1303K has been identified in the scientific literature (PMID: 29978950), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
E1400D | missense | unknown | ALK E1400D lies within the cytoplasmic domain of the Alk protein (UniProt.org). E1400D has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
E1407K | missense | unknown | ALK E1407K lies within the cytoplasmic domain of the Alk protein (UniProt.org). E1407K has been identified in the scientific literature (PMID: 29290262, PMID: 35042152), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
E310D | missense | unknown | ALK E310D lies within MAM domain 1 of the Alk protein (UniProt.org). E310D has been identified in sequencing studies (PMID: 28915720), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
E717K | missense | unknown | ALK E717K lies within the extracellular domain of the Alk protein (UniProt.org). E717K has been identified in the scientific literature (PMID: 38215117), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
E803Q | missense | unknown | ALK E803Q lies within the extracellular domain of the Alk protein (UniProt.org). E803Q has been identified in the scientific literature (PMID: 39267820), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2024). | |
E862D | missense | unknown | ALK E862D lies within the extracellular domain of the Alk protein (UniProt.org). E862D has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
E994K | missense | no effect - predicted | ALK E994K lies within the extracellular domain of the Alk protein (UniProt.org). E994K has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function. | |
F1098V | missense | unknown | ALK F1098V lies within the cytoplasmic domain of the Alk protein (UniProt.org). F1098V is not activating in an in vitro assay and is not transforming in culture (PMID: 33674381), but has not been fully biochemically characterized and therefore, its effect on Alk protein function is unknown. | |
F1174C | missense | gain of function | ALK F1174C lies within the protein kinase domain of the Alk protein (UniProt.org). F1174C confers a gain of function to Alk, as indicated by constitutive Alk phosphorylation in cell culture (PMID: 24509625, PMID: 22072639). | |
F1174I | missense | gain of function | ALK F1174I lies within the protein kinase domain of the Alk protein (UniProt.org). F1174I results in ligand-independent phosphorylation of the Alk protein, activation of Erk, Stat3 pathways, and is transforming in cell culture (PMID: 23104988). | |
F1174L | missense | gain of function | ALK F1174L lies within the protein kinase domain of the Alk protein (UniProt.org). F1174L confers a gain of function to the Alk protein as indicated by transformation activity and increased cell proliferation in culture (PMID: 18923525, PMID: 29533785, PMID: 29907598), and has been demonstrated to occur as a secondary resistance mutation in the context of ALK fusions (PMID: 21030459, PMID: 31452835). | Y |
F1174S | missense | gain of function | ALK F1174S lies within the protein kinase domain of the Alk protein (UniProt.org). F1174S results in ligand-independent activation of the Alk protein and increased Erk phosphorylation in culture (PMID: 21059859), activation in an in vitro assay (PMID: 33674381), and transformation of cells in culture (PMID: 21059859, PMID: 33674381). | |
F1174V | missense | gain of function - predicted | ALK F1174V lies within the protein kinase domain of the Alk protein (UniProt.org). F1174V results in constitutive activation of the Alk protein in cell culture (PMID: 21242967) and has been demonstrated to occur as a secondary resistance mutation in the context of ALK fusions (PMID: 24675041), and therefore, is predicted to lead to a gain of Alk protein function. | Y |
F1174X | missense | unknown | ALK F1174X indicates any Alk missense mutation that results in replacement of the phenylalanine (F) at amino acid 1174 by a different amino acid. | |
F1245C | missense | gain of function | ALK F1245C lies within the protein kinase domain of the Alk protein (UniProt.org). F1245C results in increased downstream signaling and is transforming in cell culture (PMID: 21838707, PMID: 25517749). | Y |
F1245I | missense | unknown | ALK F1245I lies within the protein kinase domain of the Alk protein (UniProt.org). F1245I has been identified in the scientific literature (PMID: 30778092, PMID: 34282791, PMID: 37523146), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
F1245L | missense | unknown | ALK F1245L lies within the protein kinase domain of the Alk protein (UniProt.org). F1245L has been identified in the scientific literature (PMID: 30867766, PMID: 18923524), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
F1245Q | missense | unknown | ALK F1245Q lies within the protein kinase domain of the Alk protein (UniProt.org). F1245Q has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
F1245V | missense | gain of function | ALK F1245V lies within the protein kinase domain of the Alk protein (UniProt.org). F1245V confers a gain of function to the Alk protein, as it results in increased Alk kinase activity in vitro and leads to transformation activity in cell culture (PMID: 25517749, PMID: 33674381, PMID: 25517749). | |
F1245X | missense | unknown | ALK F1245X indicates any Alk missense mutation that results in replacement of the phenylalanine (F) at amino acid 1245 by a different amino acid. | |
F1245Y | missense | unknown | ALK F1245Y lies within the protein kinase domain of the Alk protein (UniProt.org). F1245Y has been identified in the scientific literature (PMID: 37012551, PMID: 30523111), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
F1271L | missense | unknown | ALK F1271L lies within the protein kinase domain of the Alk protein (UniProt.org). F1271L is not activating in an in vitro assay and is not transforming in culture (PMID: 33674381), but has not been fully biochemically characterized and therefore, its effect on Alk protein function is unknown. | |
F457L | missense | no effect - predicted | ALK F457L lies within the LDL-receptor class A domain of the Alk protein (UniProt.org). F457L has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function. | |
F856S | missense | gain of function | ALK F856S lies within the extracellular domain of the Alk protein (UniProt.org). F856S confers a gain of function to the Alk protein as demonstrated by increased cell proliferation in the absence of growth factor (PMID: 26032424, PMID: 34646012) and colony formation in culture (PMID: 26032424). | |
fusion | fusion | unknown | ALK fusion indicates a fusion of the ALK gene, but the fusion partner is unknown. | |
G1123D | missense | unknown | ALK G1123D lies within the protein kinase domain of the Alk protein (UniProt.org). G1123D has been demonstrated to confer drug resistance in cell culture (PMID: 21948233), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | Y |
G1123S | missense | unknown | ALK G1123S lies within the protein kinase domain of the Alk protein (UniProt.org). G1123S has been demonstrated to confer drug resistance in culture (PMID: 26134233, PMID: 21948233), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | Y |
G1128A | missense | gain of function | ALK G1128A lies within the protein kinase domain of the Alk protein (UniProt.org). G1128A confers a gain of function to the Alk protein as demonstrated by increased activation in in vitro assays (PMID: 21838707, PMID: 33674381, PMID: 25517749) and transformation in cultured cells (PMID: 21838707, PMID: 33674381, PMID: 25517749). | |
G1128S | missense | unknown | ALK G1128S lies within the protein kinase domain of the Alk protein (UniProt.org). G1128S has been demonstrated to occur as a secondary drug resistance mutation in the context of NPM1-ALK (PMID: 25749034), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | Y |
G1137R | missense | no effect - predicted | ALK G1137R lies within the protein kinase domain of the Alk protein (UniProt.org). G1137R has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function. | |
G1201E | missense | gain of function | ALK G1201E lies within the protein kinase domain of the Alk protein (UniProt.org). G1201E confers a gain of function to the Alk protein as demonstrated by increased Alk kinase activity and downstream Pi3k and Mapk pathway activation in culture (PMID: 21596819). | |
G1201R | missense | unknown | ALK G1201R lies within the protein kinase domain of the Alk protein (UniProt.org). The functional effect of G1201R is conflicting as it is transforming (PMID: 33674381), but results in impaired kinase activity in cultured cells (PMID: 33674381, PMID: 28030793), and therefore, its effect on Alk protein function is unknown. | |
G1202del | deletion | unknown | ALK G1202del results in the deletion of an amino acid in the protein kinase domain of the Alk protein at amino acid 1202 (UniProt.org). G1202del has been demonstrated to occur as a secondary drug resistance mutation in the context of EML4-ALK (PMID: 27432227), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | Y |
G1202K | missense | unknown | ALK G1202K lies within the protein kinase domain of the Alk protein (UniProt.org). G1202K has been identified in the scientific literature (PMID: 33790576), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
G1202L | missense | unknown | ALK G1202L lies within the protein kinase domain of the Alk protein (UniProt.org). G1202L has been identified in the scientific literature (PMID: 33380260), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Apr 2024). | |
G1202R | missense | unknown | ALK G1202R lies within the protein kinase domain of the Alk protein (UniProt.org). G1202R demonstrates increased colony formation, decreased Cdh1 expression, increased migration and invasion, increased expression of metastatic factors Cdh2, Vim, Mmp2, Mmp9, and Slug in cell culture in the context of EML4-ALK (PMID: 35085771), and drug resistance in the context of ALK fusions (PMID: 22277784, PMID: 24736079, PMID: 35085771), but has not been individually characterized and therefore, its effect on Alk protein function is unknown. | Y |
G1202X | missense | unknown | ALK G1202X indicates any ALK missense mutation that results in replacement of the glycine (G) at amino acid 1202 by a different amino acid. | |
G1269A | missense | unknown | ALK G1269A lies within the protein kinase domain of the Alk protein (UniProt.org). G1269A has been demonstrated to occur as a secondary resistance mutation in the context of ALK rearrangement (PMID: 30675302, PMID: 35123209, PMID: 29872693), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | Y |
G1269E | missense | unknown | ALK G1269E lies within the protein kinase domain of the Alk protein (UniProt.org). G1269E has not been characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
G1269S | missense | gain of function - predicted | ALK G1269S lies within the protein kinase domain of the Alk protein (UniProt.org). G1269S is predicted to confer a gain of function on the Alk protein as indicated by increased Alk phosphorylation (PMID: 21948233) and has been demonstrated to confer resistance to Alk inhibitors in the context of EML4-ALK (PMID: 21948233, PMID: 22034911). | Y |
G1286R | missense | unknown | ALK G1286R lies within the protein kinase domain of the Alk protein (UniProt.org). G1286R is not activating in an in vitro assay and is not transforming in culture (PMID: 33674381, PMID: 25517749), but has not been fully biochemically characterized and therefore, its effect on Alk protein function is unknown. | |
G1552R | missense | no effect - predicted | ALK G1552R lies within the cytoplasmic domain of the Alk protein (UniProt.org). G1552R has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function. | |
G689V | missense | unknown | ALK G689V lies within the extracellular domain of the Alk protein (UniProt.org). G689V has been identified in sequencing studies (PMID: 26960398), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
G746C | missense | unknown | ALK G746C lies within the extracellular domain of the Alk protein (UniProt.org). G746C has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
G875R | missense | no effect - predicted | ALK G875R lies within the extracellular domain of the Alk protein (UniProt.org). G875R has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in culture cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function. | |
G922R | missense | unknown | ALK G922R lies within the extracellular domain of the Alk protein (UniProt.org). G922R has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
H1030P | missense | unknown | ALK H1030P lies within the extracellular domain of the ALK protein (UniProt.org). H1030P has been identified in sequencing studies (PMID: 25275298), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
H354Q | missense | no effect - predicted | ALK H354Q lies within MAM domain 1 of the Alk protein (UniProt.org). H354Q has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cell culture (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function. | |
I1170N | missense | gain of function | ALK I1170N lies within the protein kinase domain of the Alk protein (UniProt.org). I1170N confers a gain of function to Alk as demonstrated by increased kinase activity in in vitro assays and transformation in cultured cells (PMID: 33674381, PMID: 25517749). | |
I1170S | missense | gain of function | ALK I1170S lies within the protein kinase domain of the Alk protein (UniProt.org). I1170S confers a gain of function to Alk as demonstrated by increased kinase activity in in vitro assays and transformation in cultured cells (PMID: 33674381, PMID: 25517749). | |
I1170V | missense | gain of function - predicted | ALK I1170V lies within the protein kinase domain of the Alk protein (UniProt.org). I1170V is not transforming in cultured cells but is activating in an in vitro assay (PMID: 33674381), and therefore, is predicted to lead to a gain of Alk protein function. | |
I1171N | missense | gain of function | ALK I1171N lies within the protein kinase domain of the Alk protein (UniProt.org). I1171N results in increased ligand-independent Alk phosphorylation and activation of the Stat pathway in culture (PMID: 23239810, PMID: 21838707), activation in in vitro assays (PMID: 33674381, PMID: 25517749), is transforming in cell culture (PMID: 23239810, (PMID: 33674381, PMID: 25517749), and has been demonstrated to occur as a secondary resistance mutation in the context of ALK fusions (PMID: 27565911). | Y |
I1171S | missense | unknown | ALK I1171S lies within the protein kinase domain of the Alk protein (UniProt.org). I1171S has been demonstrated to confer drug resistance in the context of ALK fusions in culture (PMID: 27009859, PMID: 25393796, PMID: 26464158), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | Y |
I1171T | missense | gain of function | ALK I1171T lies within the protein kinase domain of the Alk protein (UniProt.org). I1171T confers a gain of function on the Alk protein as indicated by ligand-independent autophosphorylation, activation of Erk1/2, and cell transformation (PMID: 29907598), and has been demonstrated to confer drug resistance in the context of ALK fusions in culture (PMID: 27009859). | Y |
I1171X | missense | unknown | ALK I1171X indicates any Alk missense mutation that results in replacement of the isoleucine (I) at amino acid 1171 by a different amino acid. | |
I1179V | missense | unknown | ALK I1179V lies within the protein kinase domain of the Alk protein (UniProt.org). I1179V has been demonstrated to occur as a secondary drug resistance mutation in the context of ALK compound mutations (PMID: 29650534), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | Y |
I1183T | missense | unknown | ALK I1183T lies within the protein kinase domain of the Alk protein (UniProt.org). I1183T is not activating in an in vitro assay and is not transforming in culture (PMID: 33674381, PMID: 25517749), but has not been fully biochemically characterized and therefore, its effect on Alk protein function is unknown. | |
I1250T | missense | loss of function | ALK I1250T lies within the protein kinase domain of the Alk protein (UniProt.org). I1250T confers a loss of function on Alk as indicated by loss of kinase activity and loss of the ability to activate downstream signaling pathways in cell culture (PMID: 21804922). | |
I1268V | missense | unknown | ALK I1268V lies within the protein kinase domain of the Alk protein (UniProt.org). I1268V has been identified in the scientific literature (PMID: 31585938), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Apr 2024). | |
I1461V | missense | unknown | ALK I1461V lies within the cytoplasmic domain of the Alk protein (UniProt.org). I1461V has been identified in sequencing studies (PMID: 36422072, PMID: 36819147, PMID: 37964559), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
inact mut | unknown | loss of function | ALK inact mut indicates that this variant results in a loss of function of the Alk protein. However, the specific amino acid change has not been identified. | |
K1062M | missense | gain of function | ALK K1062M lies within the cytoplasmic domain of the Alk protein (UniProt.org). K1062M confers a gain of function to the Alk protein as demonstrated by increased Alk kinase activity (PMID: 28199182) and increased AKT and ERK phosphorylation in in vitro assays (PMID: 21596819). | |
K1491R | missense | no effect - predicted | ALK K1491R lies within the cytoplasmic domain of the Alk protein (UniProt.org). K1491R has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function. | |
K1518N | missense | no effect - predicted | ALK K1518N lies within the cytoplasmic domain of the Alk protein (UniProt.org). K1518N does not result in Alk autophosphorylation and is not transforming in culture (PMID: 22086496), and therefore, is predicted to have no effect on Alk protein function. | |
K1525E | missense | no effect - predicted | ALK K1525E lies within the cytoplasmic domain of the Alk protein (UniProt.org). K1525E does not result in Alk autophosphorylation and is not transforming in culture (PMID: 22086496), and therefore, is predicted to have no effect on Alk protein function. | |
K894T | missense | unknown | ALK K894T lies within the extracellular domain of the Alk protein (UniProt.org). K894T has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
L1122V | missense | unknown | ALK L1122V lies within the protein kinase domain of the Alk protein (UniProt.org). L1122V has been demonstrated to occur as a secondary drug resistance mutation in the context of ALK fusions (PMID: 29650534, PMID: 25421750), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | Y |
L1152M | missense | unknown | ALK L1152M lies within the protein kinase domain of the Alk protein (UniProt.org). L1152M has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
L1152P | missense | unknown | ALK L1152P lies within the protein kinase domain of the Alk protein (UniProt.org). L1152P has been demonstrated to confer drug resistance in the context of ALK rearrangement in culture (PMID: 24675041), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Apr 2024). | Y |
L1152R | missense | unknown | ALK L1152R lies within the protein kinase domain of the Alk protein (UniProt.org). L1152R has been demonstrated to occur as a secondary drug resistance mutation in the context of ALK fusions (PMID: 35123209, PMID: 36064579, PMID: 27091190), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | Y |
L1152V | missense | unknown | ALK L1152V lies within the protein kinase domain of the Alk protein (UniProt.org). L1152V is associated with decreased ALK inhibitor sensitivity in the context of an ALK fusion in culture (PMID: 22034911), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Apr 2024). | |
L1196M | missense | gain of function | ALK L1196M lies within the protein kinase domain of the Alk protein (UniProt.org). L1196M results in increased Alk kinase activity (PMID: 30258533), modest Alk autophosphorylation, and transformation in cell culture (PMID: 25517749), and confers resistance to Alk inhibitors in the context of ALK rearrangements in culture and in vivo (PMID: 21613408, PMID: 25421750, PMID: 31452835). | Y |
L1196P | missense | gain of function - predicted | ALK L1196P lies within the protein kinase domain of the Alk protein (UniProt.org). L1196P results in both increased phosphorylation of Alk and proliferation, and has been demonstrated to confer resistance to Alk inhibitors in the context of EML4-ALK in culture (PMID: 38448512), and therefore, is predicted to lead to a gain of Alk protein function. | Y |
L1196Q | missense | unknown | ALK L1196Q lies within the protein kinase domain of the Alk protein (UniProt.org). L1196Q has been demonstrated to confer resistance to Alk inhibitors in the context of ALK rearrangements (PMID: 25749034, PMID: 33209633, PMID: 38290249), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Apr 2024). | Y |
L1196X | missense | unknown | ALK L1196X indicates any Alk missense mutation that results in replacement of the leucine (L) at amino acid 1196 by a different amino acid. | |
L1198F | missense | gain of function | ALK L1198F lies within the protein kinase domain of the Alk protein (UniProt.org). L1198F confers a gain of function to the Alk protein as demonstrated by increased Alk kinase activity and downstream Pi3k and Mapk pathway activation (PMID: 21596819). | |
L1198H | missense | unknown | ALK L1198H lies within the protein kinase domain of the Alk protein (UniProt.org). L1198H has been demonstrated to occur as a secondary drug resistance mutation in the context of ALK compound mutations (PMID: 29650534), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | Y |
L1198P | missense | gain of function - predicted | ALK L1198P lies within the protein kinase domain of the Alk protein (UniProt.org). L1198P results in increased phosphorylation of Alk and has been demonstrated to confer resistance to Alk inhibitors in the context of EML4-ALK in culture (PMID: 21948233), and therefore, is predicted to lead to a gain of Alk protein function. | Y |
L1198R | missense | unknown | ALK L1198R lies within the protein kinase domain of the Alk protein (UniProt.org). L1198R has been demonstrated to occur as a secondary drug resistance mutation in the context of ALK compound mutations (PMID: 38448512), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Mar 2024). | Y |
L1198V | missense | unknown | ALK L1198V lies within the protein kinase domain of the Alk protein (UniProt.org). L1198V has been demonstrated to confer resistance to ALK inhibitors in the context of Alk fusions (PMID: 29636358, PMID: 35123209), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | Y |
L1198X | missense | unknown | ALK L1198X indicates any Alk missense mutation that results in replacement of the leucine (L) at amino acid 1198 by a different amino acid. | |
L1204F | missense | unknown | ALK L1204F lies within the protein kinase domain of the Alk protein (UniProt.org). L1204F is not activating in an in vitro assay and is not transforming in culture (PMID: 33674381, PMID: 25517749), but has not been fully biochemically characterized and therefore, its effect on Alk protein function is unknown. | |
L1204V | missense | unknown | ALK L1204V lies within the protein kinase domain of the Alk protein (UniProt.org). L1204V has been demonstrated to occur as a secondary drug resistance mutation in the context of ALK compound mutations (PMID: 29650534), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | Y |
L1256F | missense | unknown | ALK L1256F lies within the protein kinase domain of the Alk protein (UniProt.org). L1256F demonstrates transforming and tumorigenic activity and confers drug resistance in the context of ALK fusions (PMID: 29650534, PMID: 30662002), but has not been individually characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | Y |
L1319V | missense | unknown | ALK L1319V lies within the protein kinase domain of the Alk protein (UniProt.org). L1319V has been identified in the scientific literature (PMID: 33776709), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Apr 2024). | |
L560F | missense | no effect - predicted | ALK L560F lies within MAM domain 2 of the Alk protein (UniProt.org). L560F has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function. | |
L868Q | missense | unknown | ALK L868Q lies within the extracellular domain of the Alk protein (UniProt.org). L868Q has not been characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
M1166R | missense | gain of function | ALK M1166R lies within the protein kinase domain of the Alk protein (UniProt.org). M1166R confers a gain of function to the Alk protein, as indicated by ligand-independent activation of the Alk protein (PMID: 33674381, PMID: 25517749, PMID: 23104988), increased Erk and Stat3 phosphorylation in cell culture (PMID: 23104988), and transformation of cultured cells (PMID: 33674381, PMID: 25517749). | |
M1166V | missense | unknown | ALK M1166V lies within the protein kinase domain of the Alk protein (UniProt.org). M1166V has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
M1478T | missense | unknown | ALK M1478T lies within the cytoplasmic domain of the Alk protein (UniProt.org). M1478T has been identified in sequencing studies (PMID: 27852271), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
M301I | missense | no effect - predicted | ALK M301I lies within MAM domain 1 of the Alk protein (UniProt.org). M301I has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in culture cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function. | |
M552I | missense | no effect - predicted | ALK M552I lies within MAM domain 2 of the Alk protein (UniProt.org). M552I has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function. | |
mutant | unknown | unknown | ALK mutant indicates an unspecified mutation in the ALK gene. | |
N1178H | missense | unknown | ALK N1178H lies within the protein kinase domain of the Alk protein (UniProt.org). N1178H has been demonstrated to occur as a secondary drug resistance mutation in the context of NPM1-ALK (PMID: 25749034), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | Y |
negative | unknown | loss of function | ALK negative indicates a lack of expression of the ALK mRNA and/or protein. | |
over exp | none | no effect | ALK over exp indicates an over expression of the Alk protein and/or mRNA. However, the mechanism causing the over expression is unspecified. | |
P1094H | missense | unknown | ALK P1094H lies within the cytoplasmic domain of the Alk protein (UniProt.org). P1094H has been identified in the scientific literature (PMID: 33166721), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Jul 2024). | |
P1112Q | missense | unknown | ALK P1112Q lies within the cytoplasmic domain of the Alk protein (UniProt.org). P1112Q has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
P1139S | missense | unknown | ALK P1139S lies within the protein kinase domain of the Alk protein (UniProt.org). P1139S has been associated with decreased sensitivity to some ALK inhibitors in the context of NPM1-ALK (PMID: 25421750), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Apr 2024). | |
P1213C | missense | unknown | ALK P1213C lies within the protein kinase domain of the Alk protein (UniProt.org). P1213C is not activating in an in vitro assay and is not transforming in culture (PMID: 33674381), but has not been fully biochemically characterized and therefore, its effect on Alk protein function is unknown. | |
P1298S | missense | unknown | ALK P1298S lies within the protein kinase domain of the Alk protein (UniProt.org). P1298S has been identified in sequencing studies (PMID: 24755471), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
P1543S | missense | unknown | ALK P1543S lies within the cytoplasmic domain of the Alk protein (UniProt.org). P1543S has been identified in sequencing studies (PMID: 24755471), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Apr 2024). | |
P157S | missense | unknown | ALK P157S lies within the extracellular domain of the Alk protein (Uniprot.org). P157S has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
P1599S | missense | unknown | ALK P1599S lies within the cytoplasmic domain of the Alk protein (UniProt.org). P1599S has been identified in sequencing studies (PMID: 29684080), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
P279L | missense | unknown | ALK P279L lies within MAM domain 1 of the Alk protein (UniProt.org). P279L has been identified in sequencing studies (PMID: 31209238), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
P336S | missense | no effect - predicted | ALK P336S lies within MAM domain 1 of the Alk protein (UniProt.org). P336S has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function. | |
P36S | missense | unknown | ALK P36S lies within the extracellular domain of the Alk protein (UniProt.org). P36S has been identified in sequencing studies (PMID: 20579941, PMID: 26580448), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
P40L | missense | unknown | ALK P40L lies within the extracellular domain of the Alk protein (UniProt.org). P40L has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
P858S | missense | no effect - predicted | ALK P858S lies within the extracellular domain of the Alk protein (UniProt.org). P858S has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function. | |
positive | unknown | unknown | ALK positive indicates the presence of the ALK gene, mRNA, and/or protein. ALK positive has been used alternatively to refer to presence of an ALK fusion or rearrangement. For related data, refer to ALK fusion or ALK rearrange in CKB. | |
Q1146K | missense | unknown | ALK Q1146K lies within the protein kinase domain of the Alk protein (UniProt.org). Q1146K has been identified in the scientific literature (PMID: 34687488), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Apr 2024). | |
Q1146P | missense | unknown | ALK Q1146P lies within the protein kinase domain of the Alk protein (UniProt.org). Q1146P has been identified in the scientific literature (PMID: 31894386), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Apr 2024). | |
Q1188_L1190del | deletion | unknown | ALK Q1188_L1190del results in the deletion of three amino acids in the protein kinase domain of the Alk protein from amino acids 1188 to 1190 (UniProt.org). Q1188_L1190del has been identified in the scientific literature (PMID: 33887694), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
R1113Q | missense | unknown | ALK R1113Q lies within the cytoplasmic domain of the Alk protein (UniProt.org). R1113Q has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
R1181H | missense | unknown | ALK R1181H lies within the protein kinase domain of the Alk protein (UniProt.org). R1181H results in increased cytokine-independent growth and phosphorylation of Stat3, Erk, Akt, and demonstrates resistance to first generation Alk inhibitors in the context of EML4-ALK in culture (PMID: 38960389), but has not been individually characterized and therefore, its effect on Alk protein function is unknown. | Y |
R1192P | missense | gain of function | ALK R1192P lies within the protein kinase domain of the Alk protein (UniProt.org). R1192P confers a gain of function to the Alk protein as demonstrated by increased downstream signalling (PMID: 21838707), activation in in vitro assays (PMID: 33674381, PMID: 25517749), and transformation of cultured cells (PMID: 21838707, PMID: 33674381, PMID: 25517749). | |
R1192Q | missense | no effect - predicted | ALK R1192Q lies within the protein kinase domain of the Alk protein (UniProt.org). R1192Q does not result in Alk autophosphorylation and is not transforming in culture (PMID: 22086496), and therefore, is predicted to have no effect on Alk protein function. | |
R1209Q | missense | unknown | ALK R1209Q lies within the protein kinase domain of the Alk protein (UniProt.org). R1209Q leads to decreased cell proliferation and cell viability compared to wild-type Alk in one of two cell lines in culture (PMID: 29533785), but has not been biochemically characterized, and therefore, its effect on Alk protein function is unknown. | |
R1212C | missense | unknown | ALK R1212C lies within the protein kinase domain of the Alk protein (UniProt.org). R1212C is not activating in an in vitro assay and is weakly transforming in culture (PMID: 33674381), but has not been fully biochemically characterized and therefore, its effect on Alk protein function is unknown. | |
R1212H | missense | unknown | ALK R1212H lies within the protein kinase domain of the Alk protein (UniProt.org). R1212H has been identified in sequencing studies (PMID: 21499247, PMID: 28912153, PMID: 28581676), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
R1231Q | missense | unknown | ALK R1231Q lies within the protein kinase domain of the Alk protein (UniProt.org). R1231Q is not activating in an in vitro assay and is not transforming in culture (PMID: 33674381, PMID: 25517749), but has not been fully biochemically characterized and therefore, its effect on Alk protein function is unknown. | |
R1275L | missense | unknown | ALK R1275L lies within the protein kinase domain of the Alk protein (UniProt.org). R1275L has been identified in the scientific literature (PMID: 27888620), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
R1275Q | missense | gain of function | ALK R1275Q lies within the protein kinase domain of the Alk protein (UniProt.org). R1275Q confers a gain of function to the Alk protein as demonstrated by transformation activity in cell culture and increased downstream signalling in in vitro assays (PMID: 21838707, PMID: 29533785). | |
R1275X | missense | unknown | ALK R1275X indicates any Alk missense mutation that results in replacement of the arginine (R) at amino acid 1275 by a different amino acid. | |
R1279Q | missense | unknown | ALK R1279Q lies within the protein kinase domain of the Alk protein (UniProt.org). R1279Q is not transforming in culture (PMID: 33674381), but has not been fully biochemically characterized and therefore, its effect on Alk protein function is unknown. | |
R133H | missense | no effect - predicted | ALK R133H lies within the extracellular domain of the Alk protein (UniProt.org). R133H has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function. | |
R284K | missense | unknown | ALK R284K lies within MAM domain 1 of the Alk protein (UniProt.org). R284K has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
R291C | missense | unknown | ALK R291C lies within MAM domain 1 of the Alk protein (UniProt.org). R291C has been identified in sequencing studies (PMID: 24755471, PMID: 36502690), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Apr 2024). | |
R311H | missense | unknown | ALK R311H lies within MAM domain 1 of the Alk protein (UniProt.org). R311H has been identified in sequencing studies (PMID: 30410351, PMID: 23202128, PMID: 25344691), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Apr 2024). | |
R395H | missense | unknown | ALK R395H lies within MAM domain 1 of the Alk protein (UniProt.org). R395H has been identified in sequencing studies (PMID: 22877736), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Apr 2024). | |
R401* | nonsense | loss of function - predicted | ALK R401* results in a premature truncation of the Alk protein at amino acid 401 of 1620 (UniProt.org). Due to the loss of the protein kinase domain (UniProt.org), R401* is predicted to lead to a loss of Alk protein function. | |
R401L | missense | gain of function - predicted | ALK R401L lies within MAM domain 1 of the Alk protein (UniProt.org). R401L results in increased proliferation, clonogenic survival, and invasion in cultured cells (Cancer Res 2021;81(13_Suppl):Abstract nr 2422), and therefore, is predicted to lead to a gain of Alk protein function. | |
R401Q | missense | no effect - predicted | ALK R401Q lies within MAM domain 1 of the Alk protein (UniProt.org). R401Q has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted have no effect on Alk protein function. | |
R412C | missense | no effect - predicted | ALK R412C lies within MAM domain 1 of the Alk protein (UniProt.org). R412C does not result in Alk autophosphorylation and is not transforming in culture (PMID: 22086496), and therefore, is predicted to have no effect on Alk protein function. | |
R753Q | missense | gain of function - predicted | ALK R753Q lies within the extracellular domain of the Alk protein (UniProt.org). R753Q results in increased cytokine-dependent growth in cell culture (PMID: 34646012), and therefore, is predicted to lead to a gain of Alk protein function. | |
rearrange | unknown | unknown | ALK rearrangement indicates an unspecified rearrangement of the ALK gene. | |
S1053F | missense | unknown | ALK S1053F lies within the transmembrane domain of the Alk protein (UniProt.org). S1053F has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
S1189C | missense | unknown | ALK S1189C lies within the protein kinase domain of the Alk protein (UniProt.org). S1189C has been identified in the scientific literature (PMID: 36506539), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
S1206C | missense | unknown | ALK S1206C lies within the protein kinase domain of the Alk protein (UniProt.org). S1206C has been demonstrated to occur as a secondary drug resistance mutation in the context of ALK compound mutations (PMID: 25421750), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | Y |
S1206F | missense | unknown | ALK S1206F lies within the protein kinase domain of the Alk protein (UniProt.org). S1206F has been identified in the scientific literature (PMID: 27565908, PMID: 31712133, PMID: 34589977), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
S1206R | missense | unknown | ALK S1206R lies within the protein kinase domain of the Alk protein (UniProt.org). S1206R has been demonstrated to occur as a secondary drug resistance mutation in the context of EML4-ALK (PMID: 22034911), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Apr 2024). | Y |
S1206Y | missense | unknown | ALK S1206Y lies within the protein kinase domain of the Alk protein (UniProt.org). S1206Y has been demonstrated to occur as a secondary drug resistance mutation in the context of ALK rearrangements (PMID: 22277784, PMID: 24675041, PMID: 25727400), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | Y |
S529Y | missense | no effect - predicted | ALK S529Y lies within MAM domain 2 of the Alk protein (UniProt.org). S529Y has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function. | |
S711R | missense | no effect - predicted | ALK S711R lies within the extracellular domain of the Alk protein (UniProt.org). S711R has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function. | |
S865P | missense | no effect - predicted | ALK S865P lies within the extracellular domain of the Alk protein (UniProt.org). S865P has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function. | |
T1151dup | duplication | unknown | ALK T1151dup indicates the insertion of the duplicate amino acid, threonine (T)-1151, in the protein kinase domain of the Alk protein (UniProt.org). T1151dup has been demonstrated to occur as a secondary drug resistance mutation in the context of EML4-ALK (PMID: 22277784, PMID: 20695522), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | Y |
T1151K | missense | unknown | ALK T1151K lies within the protein kinase domain of the Alk protein (UniProt.org). T1151K has been demonstrated to occur as a secondary drug resistance mutation in the context of ALK fusions (PMID: 28676215, PMID: 30519133), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | Y |
T1151M | missense | gain of function - predicted | ALK T1151M lies within the protein kinase domain of the Alk protein (UniProt.org). T1151M is not transforming (PMID: 18923525, PMID: 33674381, PMID: 25517749) and does not induce Alk phosphorylation in culture (PMID: 18923525), but is activating in in vitro assays (PMID: 33674381, PMID: 25517749), and has been demonstrated to occur as a secondary drug resistance mutation in the context of ALK fusions (PMID: 28676215, PMID: 27009859), and therefore, is predicted to lead to a gain of Alk protein function. | Y |
T1151R | missense | unknown | ALK T1151R lies within the protein kinase domain of the Alk protein (UniProt.org). T1151R has been demonstrated to occur as a secondary drug resistance mutation in the context of ALK rearrangement (PMID: 31027750), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | Y |
T1343I | missense | unknown | ALK T1343I lies within the protein kinase domain of the Alk protein (UniProt.org). T1343I is not activating in an in vitro assay and is not transforming in culture (PMID: 33674381, PMID: 25517749), but has not been fully biochemically characterized and therefore, its effect on Alk protein function is unknown. | |
T429I | missense | unknown | ALK T429I lies within the extracellular domain of the Alk protein (UniProt.org). T429I has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
T535N | missense | unknown | ALK T535N lies within MAM domain 2 of the Alk protein (UniProt.org). T535N has been identified in sequencing studies (PMID: 34465320), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Apr 2024). | |
T680I | missense | no effect - predicted | ALK T680I lies within MAM domain 2 of the Alk protein (UniProt.org). T680I has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function. | |
V1180L | missense | unknown | ALK V1180L lies within the protein kinase domain of the Alk protein (UniProt.org). V1180L has been demonstrated to occur as a secondary resistance mutation in the context of EML4-ALK (PMID: 25228534, PMID: 27432227, PMID: 35324529), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | Y |
V1180X | missense | unknown | ALK V1180X indicates any Alk missense mutation that results in replacement of the valine (V) at amino acid 1180 by a different amino acid. | |
V1180Y | missense | unknown | ALK V1180Y lies within the protein kinase domain of the Alk protein (UniProt.org). V1180Y has been identified in the scientific literature (PMID: 36096442), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Apr 2024). | |
V1413G | missense | unknown | ALK V1413G lies within the cytoplasmic domain of the Alk protein (UniProt.org). V1413G has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
V1545I | missense | no effect - predicted | ALK V1545I lies within the cytoplasmic domain of the Alk protein (UniProt.org). V1545I has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function. | |
V163L | missense | unknown | ALK V163L lies within the extracellular domain of the Alk protein (UniProt.org). V163L has been identified in sequencing studies (PMID: 24728327, PMID: 34367235), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
V198M | missense | unknown | ALK V198M lies within the extracellular domain of the Alk protein (UniProt.org). V198M has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
V476A | missense | no effect - predicted | ALK V476A lies within the extracellular domain of the Alk protein (UniProt.org). V476A has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function. | |
V66A | missense | unknown | ALK V66A lies within the extracellular domain of the Alk protein (UniProt.org). V66A has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
V66G | missense | unknown | ALK V66G lies within the extracellular domain of the Alk protein (UniProt.org). V66G has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
V757M | missense | unknown | ALK V757M lies within the extracellular domain of the Alk protein (UniProt.org). V757M has been identified in the scientific literature (PMID: 37900840, PMID: 26933125, PMID: 24710307), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2024). | |
W501* | nonsense | loss of function - predicted | ALK W501* results in a premature truncation of the Alk protein at amino acid 501 of 1620 (UniProt.org). Due to the loss of the protein kinase domain (UniProt.org), W501* is predicted to lead to a loss of Alk protein function. | |
wild-type | none | no effect | Wild-type ALK indicates that no mutation has been detected within the ALK gene. | |
Y1096A | missense | unknown | ALK Y1096A lies within the cytoplasmic domain of the Alk protein (UniProt.org). Y1096A is not activating in an in vitro assay and is not transforming in culture (PMID: 33674381), but has not been fully biochemically characterized and therefore, its effect on Alk protein function is unknown. | |
Y1278A | missense | unknown | ALK Y1278A lies within the protein kinase domain of the Alk protein (UniProt.org). Y1278A is not activating in an in vitro assay but is transforming in cultured cells (PMID: 33674381), and therefore, its effect on Alk protein function is unknown. | |
Y1278E | missense | unknown | ALK Y1278E lies within the protein kinase domain of the Alk protein (UniProt.org). Y1278E is not activating in an in vitro assay and is not transforming in culture (PMID: 33674381, PMID: 25517749), but has not been fully biochemically characterized and therefore, its effect on Alk protein function is unknown. | |
Y1278S | missense | gain of function | ALK Y1278S is a hotspot mutation that lies within the activation loop of the Alk protein (PMID: 24060861, PMID: 25071110). Y1278S results in constitutive activation of Alk and is transforming in cell culture (PMID: 25517749, PMID: 29084134). | |
Y1282E | missense | unknown | ALK Y1282E lies within the protein kinase domain of the Alk protein (UniProt.org). Y1282E is not activating in an in vitro assay and is not transforming in culture (PMID: 33674381), but has not been fully biochemically characterized and therefore, its effect on Alk protein function is unknown. | |
EML4 - ALK | fusion | gain of function | EML4-ALK results from the fusion of EML4 and ALK, resulting in constitutive kinase activity, transformation in cultured cells, and tumor formation in mouse models (PMID: 19064915, PMID: 17625570, PMID: 35138907). EML4-ALK fusions have been associated with non-small cell lung cancer (PMID: 26755435). |