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Gene Symbol MAP2K1
Synonyms CFC3 | MAPKK1 | MEK1 | MEL | MKK1 | PRKMK1
Gene Description MAP2K1 (MEK1), mitogen-activated protein kinase kinase 1, is a serine-threonine kinase, which activates the RAS-RAF-MEK-ERK pathway in cell proliferation and differentiation (PMID: 22753777). MAP2K1 (MEK1) activating mutations have been identified in a number of solid tumor types including, melanoma (PMID: 29753091), colorectal (PMID: 28819429), lung adenocarcinoma (PMID: 25351745, PMID: 32361034), histiocytic sarcoma (PMID: 29768143), and Map2k1 mutations are often associated with resistance to Mek and Raf inhibition (PMID: 29753091).
NCBI Gene ID Chromosome Map Location Canonical Transcript Gene Role
5604 15 15q22.31 NM_002755 Oncogene (PMID: 30606230)

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Variant Impact Protein Effect Variant Description Associated with drug Resistance
A106T missense no effect - predicted MAP2K1 A106T lies within the protein kinase domain of the Map2k1 protein (UniProt.org). A106T results in phosphorylation of Erk (PMID: 25164765, PMID: 32641410) and transformation activity similar to wild-type Map2k1 in culture (PMID: 36442478), and proliferation similar to wild-type in a competition assay (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
A106_I107del deletion gain of function - predicted MAP2K1 A106_I107del results in the deletion of two amino acids in the protein kinase domain of the Map2k1 protein from amino acids 106 to 107 (UniProt.org). A106_I107del results in increased Erk phosphorylation in cultured cells (PMID: 32641410), and therefore, is predicted to lead to a gain of Map2k1 protein function.
A132V missense no effect - predicted MAP2K1 A132V lies within the protein kinase domain of the Map2k1 protein (UniProt.org). A132V results in proliferation similar to wild-type Map2k1 in a competition assay and transformation activity similar to wild-type in cultured cells (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
A14S missense no effect - predicted MAP2K1 A14S does not lie within any known functional domains of the Map2k1 protein (UniProt.org). A14S results in proliferation similar to wild-type Map2k1 in a competition assay and transformation activity similar to wild-type in cultured cells (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
A158T missense unknown MAP2K1 A158T lies within the protein kinase domain of the Map2k1 protein (UniProt.org). A158T has been identified in the scientific literature (PMID: 25256751), but has not been biochemically characterized and therefore, its effect on Map2k1 protein function is unknown (PubMed, Nov 2024).
A347T missense unknown MAP2K1 A347T lies within the protein kinase domain of the Map2k1 protein (UniProt.org). A347T has not been characterized in the scientific literature and therefore, its effect on Map2k1 protein function is unknown (PubMed, Sep 2024).
A390T missense unknown MAP2K1 A390T does not lie within any known functional domains of the Map2k1 protein (UniProt.org). A390T has been identified in sequencing studies (PMID: 29340109), but has not been biochemically characterized and therefore, its effect on Map2k1 protein function is unknown (PubMed, Sep 2024).
A52V missense no effect - predicted MAP2K1 A52V lies within the negative regulatory region of the Map2k1 protein (PMID: 24241536). A52V induces Erk phosphorylation similar to wild-type Map2k1 in cultured cells (PMID: 32641410, PMID: 28115009), and therefore, is predicted to have no effect on Map2k1 protein function.
A76fs frameshift loss of function - predicted MAP2K1 A76fs results in a change in the amino acid sequence of the Map2k1 protein beginning at aa 76 of 393, likely resulting in premature truncation of the functional protein (UniProt.org). A76fs has not been characterized, however, due to loss of the protein kinase domain (UniProt.org), is predicted to lead to a loss of Map2k1 protein function.
A76T missense unknown MAP2K1 A76T lies within the protein kinase domain of the Map2k1 protein (UniProt.org). A76T has not been characterized in the scientific literature and therefore, its effect on Map2k1 protein function is unknown (PubMed, Sep 2024).
A76V missense no effect - predicted MAP2K1 A76V lies within the protein kinase domain of the Map2k1 protein (UniProt.org). A76V results in proliferation similar to wild-type Map2k1 in a competition assay and transformation activity similar to wild-type in cultured cells (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
A95_R96del deletion no effect - predicted MAP2K1 A95_R96del results in the deletion of two amino acids in the protein kinase domain of the Map2k1 protein from amino acids 95 to 96 (UniProt.org). A95_R96del induces Erk phosphorylation similar to wild-type Map2k1 in cultured cells (PMID: 32641410), and therefore, is predicted to have no effect on Map2k1 protein function.
act mut unknown gain of function MAP2K1 act mut indicates that this variant results in a gain of function in the Map2k1 protein. However, the specific amino acid change has not been identified.
amp none no effect MAP2K1 amp indicates an increased number of copies of the MAP2K1 gene. However, the mechanism causing the increase is unspecified.
C121G missense unknown MAP2K1 C121G lies within the protein kinase domain of the Map2k1 protein (UniProt.org). C121G has been identified in sequencing studies (PMID: 31745173), but has not been biochemically characterized and therefore, its effect on Map2k1 protein function is unknown (PubMed, Nov 2024).
C121S missense gain of function MAP2K1 C121S lies within the protein kinase domain of the Map2k1 protein (UniProt.org). C121S confers a gain of function on the Map2k1 protein as demonstrated by increased Erk1/2 (PMID: 25899310) and Mek phosphorylation in culture and confers acquired resistance to Raf and Mek inhibitors (PMID: 21383288, PMID: 24448821, PMID: 29483135, PMID: 29753091). Y
D136N missense no effect - predicted MAP2K1 D136N lies within the protein kinase domain of the Map2k1 protein (UniProt.org). D136N results in proliferation similar to wild-type Map2k1 in a competition assay and transformation activity similar to wild-type in cultured cells (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
D136Y missense unknown MAP2K1 D136Y lies within the protein kinase domain of the Map2k1 protein (UniProt.org). D136Y has not been characterized in the scientific literature and therefore, its effect on Map2k1 protein function is unknown (PubMed, Sep 2024).
D190Y missense no effect - predicted MAP2K1 D190Y lies within the protein kinase domain of the Map2k1 protein (UniProt.org). D190Y induces Erk phosphorylation similar to wild-type Map2k1 in cultured cells (PMID: 32641410), and therefore, is predicted to have no effect on Map2k1 protein function.
D303N missense loss of function - predicted MAP2K1 D303N lies within the protein kinase domain of the Map2k1 protein (UniProt.org). D303N results in transformation activity similar to wild-type Map2k1 in cultured cells and proliferation similar to wild-type in normal serum conditions in a competition assay but decreased proliferation in a competition assay in low serum conditions (PMID: 36442478), and therefore, is predicted to lead to a loss of Map2k1 protein function.
D336H missense no effect - predicted MAP2K1 D336H lies within the protein kinase domain of the Map2k1 protein (UniProt.org). D336H results in Erk phosphorylation (PMID: 32641410) and transformation activity similar to wild-type Map2k1 in cultured cells, and proliferation similar to wild-type in a competition assay (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
D351G missense no effect - predicted MAP2K1 D351G lies within the protein kinase domain of the Map2k1 protein (UniProt.org). D351G results in proliferation similar to wild-type Map2k1 in a competition assay and transformation activity similar to wild-type in cultured cells (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
D351N missense unknown MAP2K1 D351N lies within the protein kinase domain of the Map2k1 protein (UniProt.org). D351N has been identified in sequencing studies (PMID: 25363767), but has not been biochemically characterized and therefore, its effect on Map2k1 protein function is unknown (PubMed, Sep 2024).
D65N missense gain of function - predicted MAP2K1 D65N does not lie within any known functional domains of the Map2k1 protein (UniProt.org). D65N is predicted to confer a gain of function to the Map2k1 protein as demonstrated by increased Mek phosphorylation in culture (PMID: 26566875).
D67N missense gain of function MAP2K1 D67N does not lie within any known functional domains of the Map2k1 protein (UniProt.org). D67N confers a gain of function to Map2k1 as demonstrated by increased Erk activation (PMID: 25049390, PMID: 29483135), increased Rsk phosphorylation (PMID: 25351745), and transformation in culture (PMID: 25351745, PMID: 36442478), and is associated with BRAF inhibitor resistance in the context of BRAF V600E in culture (PMID: 28986383). Y
D67Y missense gain of function - predicted MAP2K1 D67Y does not lie within any known functional domains of the Map2k1 protein (UniProt.org). D67Y results in proliferation similar to wild-type Map2k1 in a competition assay but increased transformation activity in cultured cells (PMID: 36442478), and therefore, is predicted to lead to a gain of Map2k1 protein function.
E102_I103del deletion gain of function MAP2K1 E102_I103del results in the deletion of two amino acids in the protein kinase domain of the Map2k1 protein from amino acids 102 to 103 (UniProt.org). E102_I103del confers a gain of function to the Map2k1 protein as demonstrated by activity independent of Raf and increased phosphorylation of Mek and Erk relative to wild-type Map2k1 (PMID: 29483135), increased Erk phosphorylation (PMID: 32036070, PMID: 25202140) and Mek homodimerization in culture (PMID: 30377225), increased proliferation in a competition assay, and increased transformation activity in culture (PMID: 36442478).
E114_L115del deletion no effect - predicted MAP2K1 E114_L115del results in the deletion of two amino acids in the protein kinase domain of the Map2k1 protein from amino acids 114 to 115 (UniProt.org). E114_L115del induces Erk phosphorylation similar to wild-type Map2k1 in cultured cells (PMID: 32641410), and therefore, is predicted to have no effect on Map2k1 protein function.
E120Q missense gain of function - predicted MAP2K1 E120Q lies within the protein kinase domain of the Map2k1 protein (UniProt.org). E120Q is predicted to confer a gain of function to the Map2k1 protein as demonstrated by increased Mek phosphorylation in culture (PMID: 26566875).
E144K missense gain of function MAP2K1 E144K lies within the protein kinase domain of the Map2k1 protein (UniProt.org). E144K confers a gain of function to the Map2k1 protein as demonstrated by increased Mek and Erk phosphorylation relative to wild-type Map2k1 (PMID: 26566875).
E203D missense no effect - predicted MAP2K1 E203D lies within the protein kinase domain of the Map2k1 protein (UniProt.org). E203D induces Erk phosphorylation similar to wild-type Map2k1 in cultured cells (PMID: 32641410), and therefore, is predicted to have no effect on Map2k1 protein function.
E203K missense gain of function MAP2K1 E203K lies within the protein kinase domain of the Map2k1 protein (UniProt.org). E203K confers a gain of function to the Map2k1 protein as demonstrated by constitutive Erk phosphorylation (PMID: 22197931, PMID: 29483135) and cell transformation in culture (PMID: 22197931), and is associated with drug resistance in the context of BRAF V600E in culture (PMID: 28986383). Y
E203Q missense no effect MAP2K1 E203Q lies within the protein kinase domain of the Map2k1 protein (UniProt.org). E203Q has been demonstrated to have autophosphorylation and kinase activities similar to wild-type Map2k1 in in vitro assays (PMID: 29753091).
E203V missense gain of function - predicted MAP2K1 E203V lies within the protein kinase domain of the Map2k1 protein (UniProt.org). E203V results in increased Erk phosphorylation in cultured cells (PMID: 32641410), and therefore, is predicted to lead to a gain of Map2k1 protein function.
E320A missense unknown MAP2K1 E320A lies within the protein kinase domain of the Map2k1 protein (UniProt.org). E320A has not been characterized in the scientific literature and therefore, its effect on Map2k1 protein function is unknown (PubMed, Sep 2024).
E333A missense no effect - predicted MAP2K1 E333A lies within the protein kinase domain of the Map2k1 protein (UniProt.org). E333A induces Erk phosphorylation similar to wild-type Map2k1 in cultured cells (PMID: 32641410), and therefore, is predicted to have no effect on Map2k1 protein function.
E367K missense no effect - predicted MAP2K1 E367K does not lie within any known functional domains of the Map2k1 protein (UniProt.org). E367K results in proliferation similar to wild-type Map2k1 in a competition assay and transformation activity similar to wild-type in cultured cells (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
E41_F53del deletion unknown MAP2K1 E41_F53del results in the deletion of 13 amino acids in the Map2k1 protein from amino acids 41 to 53 (UniProt.org). E41_F53del has been identified in the scientific literature (PMID: 32483240, PMID: 33289976), but has not been biochemically characterized and therefore, its effect on Map2k1 protein function is unknown (PubMed, Nov 2024).
E41_L54del deletion gain of function MAP2K1 E41_L54del results in the deletion of 14 amino acids in the Map2k1 protein from amino acids 41 to 54 (UniProt.org). E41_L54del results in increased Map2k1 nuclear localization, elevated Erk phosphorylation, and cell transformation in culture, and promotes tumorigenesis and confers resistance to ROS1 tyrosine kinase inhibitors in the context of a ROS1 fusion (PMID: 32122926). Y
E44G missense no effect MAP2K1 E44G lies within the negative regulatory region of the Map2k1 protein (PMID: 24241536). E44G does not result in constitutive and ligand-independent activation of Map2k1 or activation of Erk at levels different than wild-type Map2k1 (PMID: 28166211).
E51_Q58del deletion gain of function MAP2K1 E51_Q58del results in the deletion of eight amino acids in the Map2k1 protein from amino acids 51 to 58 (UniProt.org). E51_Q58del results in activation of Map2k1 as indicated by increased Erk and Mek phosphorylation in cell culture (PMID: 29483135).
F129L missense gain of function MAP2K1 F129L lies within the protein kinase domain of the Map2k1 protein (UniProt.org). F129L confers a gain of function to the Map2k1 protein as demonstrated by increased kinase activity compared to wild-type, activation of downstream Erk signaling, and increased binding affinity to c-Raf (PMID: 21705440) and also demonstrates resistance to some Mek and Braf inhibitors (PMID: 29753091). Y
F53C missense gain of function MAP2K1 F53C lies within the negative regulatory region of the Map2k1 protein (PMID: 24241536). F53C confers a gain of function to Map2k1 as demonstrated by increased Erk1/2 phosphorylation (PMID: 30341394) and transformation activity in cultured cells and increased proliferation in a competition assay (PMID: 36442478), and is associated with resistance to BRAF inhibitors (PMID: 30341394, PMID: 36442478). Y
F53I missense gain of function MAP2K1 F53I lies within the negative regulatory region of the Map2k1 protein (PMID: 24241536). F53I results in prolferation in low serum conditions similar to wild-type Map2k1 in a competition assay (PMID: 36442478), but confers a gain of function to Map2k1 as demonstrated by increased Erk phosphorylation in cultured cells (PMID: 32641410), increased proliferation in a competition assay in normal serum conditions, and increased transformation activity in cultured cells (PMID: 36442478).
F53L missense gain of function MAP2K1 F53L lies within the negative regulatory region of the Map2k1 protein (PMID: 24241536). F53L results in activation of Map2k1 as indicated by increased Erk and Mek phosphorylation (PMID: 25164765, PMID: 29483135), increased Rsk phosphorylation (PMID: 25351745), transformation activity in culture (PMID: 25351745, PMID: 36442478), and increased proliferation in a competition assay (PMID: 36442478), and demonstrates resistance to some Mek and Braf inhibitors (PMID: 29753091, PMID: 36442478). Y
F53S missense gain of function MAP2K1 F53S lies within the negative regulatory region of the Map2k1 protein (PMID: 24241536). F53S demonstrates phosphorylation profile similar to wild-type Map2k1 (PMID: 29753091), and results in increased Erk phosphorylation in cell culture (PMID: 16439621, PMID: 12370306).
F53V missense gain of function MAP2K1 F53V lies within the negative regulatory region of the Map2k1 protein (PMID: 24241536). F53V confers a gain of function to Map2k1 as demonstrated by increased Erk phosphorylation (PMID: 32641410) and transformation activity in cultured cells and increased proliferation in a competition assay (PMID: 36442478).
F53Y missense gain of function MAP2K1 F53Y lies within the negative regulatory region of the Map2k1 protein (PMID: 24241536). F53Y results in proliferation in low serum conditions similar to wild-type Map2k1 in a competition assay (PMID: 36442478) but confers a gain of function to Map2k1 as demonstrated by increased Erk phosphorylation (PMID: 30776432) and transformation activity in cultured cells and increased proliferation in normal serum conditions in a competition assay (PMID: 36442478).
F53_Q58del deletion gain of function MAP2K1 F53_Q58del results in the deletion of six amino acids in the Map2k1 protein from amino acids 53 to 58 (UniProt.org). F53_Q58del results in activation of Map2k1 as indicated by increased Erk and Mek phosphorylation in cell culture (PMID: 26566875, PMID: 29483135).
F53_Q58delinsL indel gain of function - predicted MAP2K1 F53_Q58delinsL results in a deletion of six amino acids of the Map2k1 protein from amino acids 53 to 58, combined with the insertion of a leucine (L) at the same site (UniProt.org). F53_Q58delinsL results in proliferation in low serum conditions similar to wild-type Map2k1 in a competition assay but results in increased transformation activity in cultured cells and increased proliferation in normal serum conditions in a competition assay (PMID: 36442478), and therefore, is predicted to lead to a gain of Map2k1 protein function.
F68L missense unknown MAP2K1 F68L lies within the protein kinase domain of the Map2k1 protein (UniProt.org). F68L has been identified in the scientific literature (PMID: 26566875), but has not been biochemically characterized and therefore, its effect on Map2k1 protein function is unknown (PubMed, Nov 2024).
fusion fusion unknown MAP2K1 fusion indicates a fusion of the MAP2K1 gene, but the fusion partner is unknown.
G128C missense unknown MAP2K1 G128C lies within the protein kinase domain of the Map2k1 protein (UniProt.org). G128C has been identified in sequencing studies (PMID: 30933438, PMID: 26960398), but has not been biochemically characterized and therefore, its effect on Map2k1 protein function is unknown (PubMed, Sep 2024).
G128D missense gain of function MAP2K1 G128D lies within the protein kinase domain of the Map2k1 protein (UniProt.org). G128D confers a gain of function to the Map2k1 protein as indicated by increased Erk phosphorylation in culture (PMID: 28115009, PMID: 25899310, PMID: 34726260).
G128N missense no effect - predicted MAP2K1 G128N lies within the protein kinase domain of the Map2k1 protein (UniProt.org). G128N results in autophosphorylation levels similar to wild-type Map2k1 in an in vitro assay (PMID: 29753091), and therefore, is predicted to have no effect on Map2k1 protein function.
G128R missense unknown MAP2K1 G128R lies within the protein kinase domain of the Map2k1 protein (UniProt.org). G128R has been identified in sequencing studies (PMID: 30933438), but has not been biochemically characterized and therefore, its effect on Map2k1 protein function is unknown (PubMed, Sep 2024).
G128V missense unknown MAP2K1 G128V lies within the protein kinase domain of the Map2k1 protein (UniProt.org). G128V results in autophosphorylation levels similar to wild-type Map2k1 in an in vitro assay (PMID: 29753091) and has been described as a drug resistance mutation (PMID: 24265153), but leads to increased Erk phosphorylation, enhanced cell cycle progression, increased colony formation, and elevated autophagy induction compared to wild-type Map2k1 in culture (PMID: 31972311), and therefore, its effect on Map2k1 protein function is unknown. Y
G176S missense no effect - predicted MAP2K1 G176S lies within the protein kinase domain of the Map2k1 protein (UniProt.org). G176S results in proliferation similar to wild-type Map2k1 in a competition assay and transformation activity similar to wild-type in cultured cells (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
G276W missense gain of function - predicted MAP2K1 G276W lies within the protein kinase domain of the Map2k1 protein (UniProt.org). G276W results in transformation activity similar to wild-type Map2k1 in cultured cells and proliferation in normal serum conditions similar to wild-type in a competition assay but increased proliferation in a competition assay in low serum conditions (PMID: 36442478), and therefore, is predicted to lead to a gain of Map2k1 protein function.
G294E missense no effect - predicted MAP2K1 G294E lies within the protein kinase domain of the Map2k1 protein (UniProt.org). G294E results in proliferation similar to wild-type Map2k1 in a competition assay and transformation activity similar to wild-type in cultured cells (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
G294R missense no effect - predicted MAP2K1 G294R lies within the protein kinase domain of the Map2k1 protein (UniProt.org). G294R induces Erk phosphorylation similar to wild-type Map2k1 in cultured cells (PMID: 32641410), and therefore, is predicted to have no effect on Map2k1 protein function.
G301* nonsense unknown MAP2K1 G301* results in a premature truncation of the Map2k1 protein at amino acid 301 of 393 (UniProt.org). G301* has not been characterized in the scientific literature and therefore, its effect on Map2k1 protein function is unknown (PubMed, Nov 2024).
G61_D65del deletion gain of function MAP2K1 G61_D65del results in the deletion of five amino acids in the Map2k1 protein from amino acids 61 to 65 (UniProt.org). G61_D65del confers a gain of function to Map2k1 as demonstrated by increased proliferation in a competition assay and increased transformation activity in cultured cells (PMID: 36442478).
G79V missense no effect - predicted MAP2K1 G79V lies within the protein kinase domain of the Map2k1 protein (UniProt.org). G79V results in proliferation similar to wild-type Map2k1 in a competition assay and transformation activity similar to wild-type in cultured cells (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
G80S missense no effect - predicted MAP2K1 G80S lies within the protein kinase domain of the Map2k1 protein (UniProt.org). G80S results in proliferation similar to wild-type Map2k1 in a competition assay and transformation activity similar to wild-type in cultured cells (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
H100_I103delinsPL indel gain of function - predicted MAP2K1 H100_I103delinsPL results in a deletion of four amino acids of the Map2k1 protein from amino acids 100 to 103, combined with the insertion of a proline (P) and a leucine (L) at the same site (UniProt.org). H100_I103delinsPL results in proliferation under low serum conditions similar to wild-type Map2k1 in a competition assay but is predicted to lead to a gain of Map2k1 protein function as demonstrated by increased proliferation in a competition assay and increased transformation activity in cultured cells and resistance to Braf inhibitors in the context of BRAF V600E in culture (PMID: 36442478).
H100_L101del deletion no effect - predicted MAP2K1 H100_L101del results in the deletion of two amino acids in the protein kinase domain of the Map2k1 protein from amino acids 100 to 101 (UniProt.org). H100_L101del induces Erk phosphorylation similar to wild-type Map2k1 in cultured cells (PMID: 32641410), and therefore, is predicted to have no effect on Map2k1 protein function.
H119P missense unknown MAP2K1 H119P lies within the protein kinase domain of the Map2k1 protein (UniProt.org). H119P has been described as a drug resistance mutation (PMID: 19915144), but has not been biochemically characterized and therefore, its effect on Map2k1 protein function is unknown (PubMed, Sep 2024). Y
H119Q missense gain of function - predicted MAP2K1 H119Q lies within the protein kinase domain of the Map2k1 protein (UniProt.org). H119Q results in increased Erk phosphorylation in cultured cells (PMID: 32641410), and therefore, is predicted to lead to a gain of Map2k1 protein function.
H119Y missense gain of function - predicted MAP2K1 H119Y lies within the protein kinase domain of the Map2k1 protein (UniProt.org). H119Y results in increased Erk phosphorylation in cultured cells (PMID: 32641410), and therefore, is predicted to lead to a gain of Map2k1 protein function.
I103N missense gain of function MAP2K1 I103N lies within the protein kinase domain of the Map2k1 protein (UniProt.org). I103N confers a gain of function to the Map2k1 protein as demonstrated by elevated basal kinase activity in an in vitro assay (PMID: 12370306), increased Map2k1 autophosphorylation (PMID: 29753091), and transformation activity in cell culture and increased proliferation in a competition assay (PMID: 36442478), and is also associated with resistance to Mek inhibitors (PMID: 12370306, PMID: 19915144). Y
I103_K104del deletion gain of function MAP2K1 I103_K104del results in the deletion of two amino acids in the Map2k1 protein from amino acids 103 to 104 (UniProt.org). I103_K104del confers a gain of function to the Map2k1 protein as demonstrated by activity independent of Raf and increased phosphorylation of Mek and Erk relative to wild-type Map2k1 (PMID: 29483135), increased Mek and Erk phosphorylation in culture (PMID: 28935960, PMID: 30377225), increased proliferation in a competition assay, and increased transformation activity in culture (PMID: 36442478).
I104_P105del deletion gain of function - predicted MAP2K1 I104_P105del results in the deletion of two amino acids in the protein kinase domain of the Map2k1 protein from amino acids 104 to 105 (UniProt.org). I104_P105del results in increased Erk phosphorylation in cultured cells (PMID: 32641410), and therefore, is predicted to lead to a gain of Map2k1 protein function.
I107_R108del deletion gain of function - predicted MAP2K1 I107_R108del results in the deletion of two amino acids in the protein kinase domain of the Map2k1 protein from amino acids 107 to 108 (UniProt.org). I107_R108del results in increased Erk phosphorylation in cultured cells (PMID: 32641410), and therefore, is predicted to lead to a gain of Map2k1 protein function.
I111A missense gain of function - predicted MAP2K1 I111A lies within the protein kinase domain of the Map2k1 protein (UniProt.org). I111A is predicted to confer a gain of function to the Map2k1 protein as demonstrated by increased basal Map2k1 kinase activity in an in vitro assay and is associated with decreased sensitivity to Mek inhibition (PMID: 12370306).
I111D missense gain of function - predicted MAP2K1 I111D lies within the protein kinase domain of the Map2k1 protein (UniProt.org). I111D is predicted to confer a gain of function to the Map2k1 protein as demonstrated by increased basal Map2k1 kinase activity in an in vitro assay and is associated with resistance to Mek inhibition (PMID: 12370306). Y
I111N missense gain of function - predicted MAP2K1 I111N lies within the protein kinase domain of the Map2k1 protein (UniProt.org). I111N is predicted to confer a gain of function to the Map2k1 protein as demonstrated by increased Mek and Erk phosphorylation in cultured cells (PMID: 19915144), and is also associated with resistance to Mek inhibitors (PMID: 19915144, PMID: 28655712). Y
I111P missense gain of function - predicted MAP2K1 I111P lies within the protein kinase domain of the Map2k1 protein (UniProt.org). I111P is predicted to confer a gain of function to the Map2k1 protein as demonstrated by increased basal Map2k1 kinase activity in an in vitro assay and is also associated with resistance to Mek inhibition (PMID: 12370306). Y
I111R missense gain of function - predicted MAP2K1 I111R lies within the protein kinase domain of the Map2k1 protein (UniProt.org). I111R is predicted to confer a gain of function to the Map2k1 protein as demonstrated by increased basal Map2k1 kinase activity in an in vitro assay and is also associated with resistance to Mek inhibition (PMID: 12370306). Y
I111S missense gain of function MAP2K1 I111S lies within the protein kinase domain of the Map2k1 protein (UniProt.org). I111S confers a gain of function on the Map2k1 protein as demonstrated by increased autophosphorylation and Erk phosphorylation and also demonstrates resistance to some Mek and Braf inhibitors (PMID: 29753091). Y
I111_I112del deletion gain of function - predicted MAP2K1 I111_I112del results in the deletion of two amino acids in the protein kinase domain of the Map2k1 protein from amino acids 111 to 112 (UniProt.org). I111_I112del results in increased Erk phosphorylation in cultured cells (PMID: 32641410), and therefore, is predicted to lead to a gain of Map2k1 protein function.
I112_R113del deletion gain of function - predicted MAP2K1 I112_R113del results in the deletion of two amino acids in the protein kinase domain of the Map2k1 protein from amino acids 112 to 113 (UniProt.org). I112_R113del results in increased Erk phosphorylation in cultured cells (PMID: 32641410), and therefore, is predicted to lead to a gain of Map2k1 protein function.
I139G missense no effect - predicted MAP2K1 I139G lies within the protein kinase domain of the Map2k1 protein (UniProt.org). I139G is predicted to have no effect on Map2k1 protein function as demonstrated by basal kinase activity similar to wild-type Map2k1 in an in vitro assay, and is associated with resistance to Mek inhibition (PMID: 12370306). Y
I139N missense no effect - predicted MAP2K1 I139N lies within the protein kinase domain of the Map2k1 protein (UniProt.org). I139N is predicted to have no effect on Map2k1 protein function as demonstrated by basal kinase activity similar to wild-type Map2k1 in an in vitro assay, and is associated with decreased sensitivity to Mek inhibition (PMID: 12370306).
I139R missense no effect - predicted MAP2K1 I139R lies within the protein kinase domain of the Map2k1 protein (UniProt.org). I139R is predicted to have no effect on Map2k1 protein function as demonstrated by basal kinase activity similar to wild-type Map2k1 in an in vitro assay, and is associated with decreased sensitivity to Mek inhibition (PMID: 12370306).
I141S missense unknown MAP2K1 I141S lies within the protein kinase domain of the Map2k1 protein (UniProt.org). I141S has been demonstrated to confer resistance to Braf inhibitors in the context of BRAF G469V (PMID: 34737188), but has not been biochemically characterized and therefore, its effect on Map2k1 protein function is unknown (PubMed, Jul 2024). Y
I204T missense gain of function MAP2K1 I204T lies within the protein kinase domain of the Map2k1 protein (UniProt.org). I204T confers a gain of function to the Map2k1 protein as indicated by transformation activity and increased phosphorylation of Erk1/2 in culture (PMID: 22327936).
I310L missense no effect - predicted MAP2K1 I310L lies within the protein kinase domain of the Map2k1 protein (UniProt.org). I310L results in Erk phosphorylation (PMID: 32641410) and transformation activity similar to wild-type Map2k1 in cultured cells, and proliferation similar to wild-type in a competition assay (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
I99G missense gain of function - predicted MAP2K1 I99G lies within the protein kinase domain of the Map2k1 protein (UniProt.org). I99G results in increased Erk2 phosphorylation and confers resistance to some Mek and Braf inhibitors in culture (PMID: 31925410), and therefore, is predicted to lead to a gain of Map2k1 protein function. Y
I99M missense unknown MAP2K1 I99M lies within the protein kinase domain of the Map2k1 protein (UniProt.org). I99M has been identified in the scientific literature (PMID: 31925410), but has not been biochemically characterized and therefore, its effect on Map2k1 protein function is unknown (PubMed, Sep 2024).
I99T missense gain of function - predicted MAP2K1 I99T lies within the protein kinase domain of the Map2k1 protein (UniProt.org). I99T results in increased Erk2 phosphorylation and confers resistance to some Mek and Braf inhibitors in culture (PMID: 31925410), and therefore, is predicted to lead to a gain of Map2k1 protein function. Y
I99_K104del deletion gain of function MAP2K1 I99_K104del results in the deletion of six amino acids in the Map2k1 protein from amino acids 99 to 104 (UniProt.org). I99_K104del confers a gain of function to the Map2k1 protein as demonstrated by Raf-independent activity, increased phosphorylation of Mek and Erk (PMID: 29483135), increased proliferation in a competition assay, and increased transformation in cultured cells (PMID: 36442478).
I9Sfs*3 frameshift loss of function - predicted MAP2K1 I9Sfs*3 indicates a shift in the reading frame starting at amino acid 9 and terminating 3 residues downstream causing a premature truncation of the 393 amino acid Map2k1 protein (UniProt.org). Due to the loss of the protein kinase domain (UniProt.org), I9Sfs*3 is predicted to lead to a loss of Map2k1 protein function.
inact mut unknown loss of function MAP2K1 inact mut indicates that this variant results in a loss of function of the Map2k1 protein. However, the specific amino acid change has not been identified.
K35N missense no effect - predicted MAP2K1 K35N does not lie within any known functional domains of the Map2k1 protein (UniProt.org). K35N induces Erk phosphorylation similar to wild-type Map2k1 in cultured cells (PMID: 32641410), and therefore, is predicted to have no effect on Map2k1 protein function.
K4N missense no effect - predicted MAP2K1 K4N does not lie within any known functional domains of the Map2k1 protein (UniProt.org). K4N results in proliferation similar to wild-type Map2k1 in a competition assay and transformation activity similar to wild-type in cultured cells (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
K57C missense gain of function - predicted MAP2K1 K57C lies within the negative regulatory region of the Map2k1 protein (PMID: 24241536). K57C has not been biochemically characterized, however, results in restored pathway activation in the presence of an upstream pathway inhibitor (PMID: 24463458), and therefore, is predicted to lead to a gain of Map2k1 protein function.
K57E missense gain of function MAP2K1 K57E lies within the negative regulatory region of the Map2k1 protein (PMID: 24241536). K57E confers a gain of function to Map2k1 as demonstrated by increased activation of downstream Erk1/2 (PMID: 24463458, PMID: 25370473) and increased transformation in cultured cells and increased proliferation in a competition assay (PMID: 36442478), and is associated with Braf inhibitor resistance in cell culture (PMID: 24463458, PMID: 25370473, PMID: 36442478). Y
K57M missense gain of function MAP2K1 K57M lies within the negative regulatory region of the Map2k1 protein (PMID: 24241536). K57M results in increased Erk phosphorylation, enhanced cell cycle progression, increased colony formation, and induction of autophagy in cultured cells (PMID: 31972311).
K57N missense gain of function MAP2K1 K57N lies within the negative regulatory region of the Map2k1 protein (PMID: 24241536). K57N confers a gain of function to Map2k1 as demonstrated by increased autophosphorylation (PMID: 29753091) and Erk phosphorylation (PMID: 18632602, PMID: 29753091), increased cell proliferation and cell viability in two different cell lines (PMID: 29533785, PMID: 18632602), transformation of cultured cells (PMID: 25351745, PMID: 36442478), increased proliferation in a competition assay (PMID: 36442478), and demonstrates resistance to some Mek and Braf inhibitors (PMID: 29753091, PMID: 36442478). Y
K57T missense gain of function MAP2K1 K57T lies within the negative regulatory region of the Map2k1 protein (PMID: 24241536). K57T confers a gain of function to Map2k1 as demonstrated by increased Erk1/2 phosphorylation (PMID: 30341394, PMID: 32641410) and transformation activity in cultured cells and increased proliferation in a competition assay (PMID: 36442478), and occurs as a secondary drug resistance mutation in the context of BRAF inhibitors (PMID: 26644315, PMID: 28819429, PMID: 30341394). Y
K57_G61del deletion gain of function - predicted MAP2K1 K57_G61del results in the deletion of five amino acids of the Map2k1 protein from amino acids 57 to 61 (UniProt.org). K57_G61del results in increased Erk phosphorylation in cultured cells (PMID: 32641410), and therefore, is predicted to lead to a gain of Map2k1 protein function.
K59del deletion unknown MAP2K1 K59del results in the deletion of an amino acid in the protein kinase domain of the Map2k1 protein at amino acid 59 (UniProt.org). K59del results in increased Mek and Erk phosphorylation in culture (PMID: 22389471), but demonstrates autophosphorylation levels similar to wild-type Map2k1 in another study (PMID: 29753091), and therefore, its effect on Map2k1 protein function is unknown.
K97M missense loss of function MAP2K1 K97M lies within the protein kinase domain of the Map2k1 protein (UniProt.org). K97M is referred to as a kinase dead variant and does not result in phosphorylation of Erk1/2 and therefore, confers a loss of function to the Map2k1 protein (PMID: 15342384).
K97_L98del deletion no effect - predicted MAP2K1 K97_L98del results in the deletion of two amino acids in the protein kinase domain of the Map2k1 protein from amino acids 97 to 98 (UniProt.org). K97_L98del induces Erk phosphorylation similar to wild-type Map2k1 in cultured cells (PMID: 32641410), and therefore, is predicted to have no effect on Map2k1 protein function.
L101_E102del deletion gain of function - predicted MAP2K1 L101_E102del results in the deletion of two amino acids in the protein kinase domain of the Map2k1 protein from amino acids 101 to 102 (UniProt.org). L101_E102del results in increased Erk phosphorylation in cultured cells (PMID: 32641410), and therefore, is predicted to lead to a gain of Map2k1 protein function.
L115A missense gain of function - predicted MAP2K1 L115A lies within the protein kinase domain of the Map2k1 protein (UniProt.org). L115A is predicted to confer a gain of function to the Map2k1 protein, as demonstrated by increased kinase activity in an in vitro assay (PMID: 12370306) and is also associated with decreased binding and partial response to Mek inhibitors (PMID: 12370306).
L115P missense gain of function MAP2K1 L115P lies within the protein kinase domain of the Map2k1 protein (UniProt.org). L115P confers a gain of function on the kinase activity of the Map2k1 protein, as demonstrated by increased autophosphorylation in cell culture (PMID: 29753091) and increased basal kinase activity in an in vitro assay (PMID: 12370306), and is also associated with decreased binding and resistance to Mek inhibitors (PMID: 12370306, PMID: 19915144, PMID: 26399658). Y
L115R missense unknown MAP2K1 L115R lies within the protein kinase domain of the Map2k1 protein (UniProt.org). L115R has been described as a drug resistance mutation (PMID: 19915144), but has not been biochemically characterized and therefore, its effect on Map2k1 protein function is unknown (PubMed, Sep 2024). Y
L118D missense loss of function - predicted MAP2K1 L118D lies within the protein kinase domain of the Map2k1 protein (UniProt.org). L118D is predicted to confer a loss of function to the Map2k1 protein as demonstrated by decreased basal Map2k1 kinase activity in an in vitro assay and is associated with resistance to Mek inhibition (PMID: 12370306). Y
L118G missense loss of function - predicted MAP2K1 L118G lies within the protein kinase domain of the Map2k1 protein (UniProt.org). L118G is predicted to confer a loss of function to the Map2k1 protein as demonstrated by decreased basal Map2k1 kinase activity in an in vitro assay and is associated with decreased sensitivity to Mek inhibition (PMID: 12370306).
L118P missense loss of function - predicted MAP2K1 L118P lies within the protein kinase domain of the Map2k1 protein (UniProt.org). L118P is associated with MEK inhibitor resistance and results in decreased basal kinase activity in an in vitro assay (PMID: 12370306), and therefore, its predicted to lead to a loss of Map2k1 protein function. Y
L118Q missense loss of function - predicted MAP2K1 L118Q lies within the protein kinase domain of the Map2k1 protein (UniProt.org). L118Q is predicted to confer a loss of function to the Map2k1 protein as demonstrated by decreased basal Map2k1 kinase activity in an in vitro assay and is associated with resistance to Mek inhibition (PMID: 12370306). Y
L177M missense gain of function MAP2K1 L177M lies within the protein kinase domain of the Map2k1 protein (UniProt.org). L177M confers a gain of function to the Map2k1 protein as indicated by a moderate increase in basal Erk signaling, sensitivity to Raf pathway activation (PMID: 29483135), and increased Erk phosphorylation in cultured cells (PMID: 32641410).
L177V missense gain of function - predicted MAP2K1 L177V lies within the protein kinase domain of the Map2k1 protein (UniProt.org). L177V results in weak phosphorylation of Erk (PMID: 32641410, PMID: 29483135) and phosphorylation of Mek in culture (PMID: 29483135), and therefore, is predicted to lead to a gain of map2k1 protein function.
L215F missense no effect - predicted MAP2K1 L215F lies within the protein kinase domain of the Map2k1 protein (UniProt.org). L215F results in proliferation similar to wild-type Map2k1 in a competition assay and transformation activity similar to wild-type in cultured cells (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
L235H missense no effect - predicted MAP2K1 L235H lies within the protein kinase domain of the Map2k1 protein (UniProt.org). L235H results in Erk phosphorylation (PMID: 32641410) and transformation activity similar to wild-type Map2k1 in culture (PMID: 36442478) and proliferation similar to wild-type in a competition assay (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
L313F missense no effect - predicted MAP2K1 L313F lies within the protein kinase domain of the Map2k1 protein (UniProt.org). L313F induces Erk phosphorylation similar to wild-type Map2k1 in cultured cells (PMID: 32641410), and therefore, is predicted to have no effect on Map2k1 protein function.
L37P missense gain of function MAP2K1 L37P lies within the nuclear export region of the Map2k1 protein (PMID: 26566875). L37P confers a gain of function to the Map2k1 protein as demonstrated by increased Mek and Erk phosphorylation relative to wild-type Map2k1 (PMID: 26566875).
L42F missense gain of function - predicted MAP2K1 L42F lies within the negative regulatory region of the Map2k1 protein (PMID: 24241536). L42F results in autophosphorylation and kinase activity similar to wild-type Map2k1 in in vitro assays (PMID: 29753091), proliferation similar to wild-type in a competition assay, but increased transformation activity (PMID: 36442478) and increased Erk phosphorylation in cultured cells (PMID: 32641410), and therefore, is predicted to lead to a gain of Map2k1 protein function.
L42_K57del deletion gain of function MAP2K1 L42_K57del results in the deletion of 16 amino acids in the Map2k1 protein from amino acids 42 to 57 (UniProt.org). L42_K57del confers a gain of function to Map2k1 as demonstrated by increased proliferation in a competition assay and increased transformation activity in cultured cells (PMID: 36442478).
L54P missense gain of function - predicted MAP2K1 L54P does not lie within any known functional domains of the Map2k1 protein (UniProt.org). L54P results in proliferation similar to wild-type Map2k1 in a competition assay but increased transformation activity in cultured cells (PMID: 36442478), and therefore, is predicted to lead to a gain of Map2k1 protein function.
L63_D67del deletion gain of function MAP2K1 L63_D67del results in the deletion of five amino acids in the Map2k1 protein from amino acids 63 to 67 (UniProt.org). L63_D67del confers a gain of function to Map2k1 as demonstrated by increased proliferation in a competition assay and increased transformation activity in cultured cells (PMID: 36442478).
L98_I103del deletion gain of function MAP2K1 L98_I103del results in the deletion of six amino acids in the Map2k1 protein from amino acids 98 to 103 (UniProt.org). L98_I103del confers a gain of function to the Map2k1 protein as demonstrated by activity independent of Raf and increased phosphorylation of Mek and Erk relative to wild-type Map2k1 in vitro (PMID: 29483135).
L98_K104delinsQ indel gain of function MAP2K1 L98_K104delinsQ results in the deletion of seven amino acids in the protein kinase domain of the Map2k1 protein from amino acids 98 to 104, combined with the insertion of a glutamine (Q) at the same site (UniProt.org). L98_K104delinsQ results in increased activation of Map2k1 and elevated phosphorylation of Erk and Akt in culture (PMID: 29768711), and has been demonstrated to confer resistance to Mek inhibitors (PMID: 29768711). Y
M146I missense no effect - predicted MAP2K1 M146I lies within the protein kinase domain of the Map2k1 protein (UniProt.org). M146I results in Erk phosphorylation (PMID: 32641410), proliferation in a competition assay, and transformation activity similar to wild-type Map2k1 in cultured cells (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
M146T missense no effect - predicted MAP2K1 M146T lies within the protein kinase domain of the Map2k1 protein (UniProt.org). M146T induces Erk phosphorylation similar to wild-type Map2k1 in cultured cells (PMID: 32641410), and therefore, is predicted to have no effect on Map2k1 protein function.
M94I missense no effect - predicted MAP2K1 M94I lies within the protein kinase domain of the Map2k1 protein (UniProt.org). M94I results in proliferation similar to wild-type Map2k1 in a competition assay and transformation activity similar to wild-type in cultured cells (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
mutant unknown unknown MAP2K1 mutant indicates an unspecified mutation in the MAP2K1 gene.
N109_Q110del deletion gain of function - predicted MAP2K1 N109_Q110del results in the deletion of two amino acids in the protein kinase domain of the Map2k1 protein from amino acids 109 to 110 (UniProt.org). N109_Q110del results in increased Erk phosphorylation in cultured cells (PMID: 32641410), and therefore, is predicted to lead to a gain of Map2k1 protein function.
N109_R113del deletion gain of function MAP2K1 N109_R113del results in the deletion of five amino acids in the protein kinase domain of the Map2k1 protein from amino acids 109 to 113 (UniProt.org). N109_R113del confers a gain of function to the Map2k1 protein as indicated by increased Erk phosphorylation (PMID: 32641410, PMID: 30393068), and Mek phosphorylation in cultured cells (PMID: 30393068).
N122D missense gain of function - predicted MAP2K1 N122D lies within the protein kinase domain of the Map2k1 protein (UniProt.org). N122D results in increased Erk phosphorylation in cultured cells (PMID: 32641410), and therefore, is predicted to lead to a gain of Map2k1 protein function.
N345T missense no effect - predicted MAP2K1 N345T lies within the protein kinase domain of the Map2k1 protein (UniProt.org). N345T results in proliferation similar to wild-type Map2k1 in a competition assay and transformation activity similar to wild-type in cultured cells (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
N382H missense no effect - predicted MAP2K1 N382H does not lie within any known functional domains of the Map2k1 protein (UniProt.org). N382H does not induce Erk phosphorylation in cultured cells (PMID: 22197931) and results in slower cell proliferation compared to wild-type Map2k1 in culture (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
P105_A106del deletion gain of function MAP2K1 P105_A106del results in the deletion of two amino acids in the protein kinase domain of the Map2k1 protein from amino acids 105 to 106 (UniProt.org). P105_A106del results in increased phosphorylation of Mek and Erk relative to wild-type Map2k1 in cell culture (PMID: 30377225).
P105_I107del deletion unknown MAP2K1 P105_I107del results in the deletion of three amino acids in the protein kinase domain of the Map2k1 protein from amino acids 105 to 107 (UniProt.org). P105_I107del has been identified in the scientific literature (PMID: 30867592), but has not been biochemically characterized and therefore, its effect on Map2k1 protein function is unknown (PubMed, Nov 2024).
P105_I107delinsL indel gain of function - predicted MAP2K1 P105_I107delinsL results in the deletion of three amino acids in the protein kinase domain of the Map2k1 protein from amino acids 105 to 107, combined with the insertion of a leucine (L) at the same site (UniProt.org). P105_I107delinsL results in increased Erk phosphorylation in cultured cells (PMID: 32641410), and therefore, is predicted to lead to a gain of Map2k1 protein function.
P124L missense gain of function MAP2K1 P124L lies within the protein kinase domain of the Map2k1 protein (UniProt.org). P124L confers a gain of function on the Map2k1 protein as demonstrated by Raf-dependent activation of Erk in an in vitro assay and moderately increased Erk and Mek phosphorylation in cultured cells (PMID: 29483135) and confers resistance to some Mek and Braf inhibitors in the context of BRAF V600 mutations in cultured cells (PMID: 19915144). Y
P124M missense unknown MAP2K1 P124M lies within the protein kinase domain of the Map2k1 protein (UniProt.org). P124M has not been characterized in the scientific literature and therefore, its effect on Map2k1 protein function is unknown (PubMed, Jul 2024).
P124Q missense unknown MAP2K1 P124Q lies within the protein kinase domain of the Map2k1 protein (UniProt.org). P124Q results in increased Erk1/2 phosphorylation in culture, and is associated with decreased sensitivity to Raf inhibition (PMID: 25370473), but demonstrates autophosphorylation and kinase activity similar to wild-type Map2k1 in another study (PMID: 29753091), and therefore, its effect on Map2k1 protein function is unknown.
P124R missense gain of function - predicted MAP2K1 P124R lies within the protein kinase domain of the Map2k1 protein (UniProt.org). P124R results in increased Erk phosphorylation in cultured cells (PMID: 32641410), and therefore, is predicted to lead to a gain of Map2k1 protein function.
P124S missense unknown MAP2K1 P124S lies within the protein kinase domain of the Map2k1 protein (UniProt.org). P124S results in increased Erk1/2 phosphorylation and confers resistance to Mek and Braf inhibitors (PMID: 19915144, PMID: 22197931) and an Egfr antibody (PMID: 33322618) in culture, but has similar induction of cell proliferation and viability levels to wild-type Map2k1 in culture (PMID: 29533785), and therefore, its effect on Map2k1 protein function is unknown. Y
P162S missense no effect - predicted MAP2K1 P162S lies within the protein kinase domain of the Map2k1 protein (UniProt.org). P162S results in proliferation similar to wild-type Map2k1 in a competition assay and transformation activity similar to wild-type in cultured cells (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
P193S missense no effect - predicted MAP2K1 P193S lies within the protein kinase domain of the Map2k1 protein (UniProt.org). P193S results in Erk phosphorylation (PMID: 32641410), proliferation in a competition assay, and transformation activity similar to wild-type in cultured cells (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
P264L missense unknown MAP2K1 P264L lies within the protein kinase domain of the Map2k1 protein (UniProt.org). P264L has been demonstrated to confer resistance to Raf inhibitors in the context of BRAF V600E in culture (PMID: 28986383), but has not been biochemically characterized and therefore, its effect on Map2k1 protein function is unknown (PubMed, Sep 2024). Y
P264R missense no effect - predicted MAP2K1 P264R lies within the protein kinase domain of the Map2k1 protein (UniProt.org). P264R induces Erk phosphorylation similar to wild-type Map2k1 in cultured cells (PMID: 32641410), and therefore, is predicted to have no effect on Map2k1 protein function.
P264S missense no effect - predicted MAP2K1 P264S lies within the protein kinase domain of the Map2k1 protein (UniProt.org). P264S results in Erk phosphorylation (PMID: 32641410) and transformation activity similar to wild-type Map2k1 in culture (PMID: 36442478) and proliferation similar to wild-type in a competition assay (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
P293S missense no effect - predicted MAP2K1 P293S lies within the protein kinase domain of the Map2k1 protein (UniProt.org). P293S results in proliferation similar to wild-type Map2k1 in a competition assay and transformation activity similar to wild-type in cultured cells (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
P306H missense gain of function MAP2K1 P306H lies within the protein kinase domain of the Map2k1 protein (UniProt.org). P306H confers a gain of function to the Map2k1 protein as indicated by increased phosphorylation of Erk1/2 and modest transformation activity in culture (PMID: 22327936).
P321H missense no effect - predicted MAP2K1 P321H lies within the protein kinase domain of the Map2k1 protein (UniProt.org). P321H induces Erk phosphorylation similar to wild-type Map2k1 in cultured cells (PMID: 32641410), and therefore, is predicted to have no effect on Map2k1 protein function.
P323A missense no effect - predicted MAP2K1 P323A lies within the protein kinase domain of the Map2k1 protein (UniProt.org). P323A induces Erk phosphorylation similar to wild-type Map2k1 in cultured cells (PMID: 32641410), and therefore, is predicted to have no effect on Map2k1 protein function.
P326H missense no effect - predicted MAP2K1 P326H lies within the protein kinase domain of the Map2k1 protein (UniProt.org). P326H results in proliferation similar to wild-type Map2k1 in a competition assay and transformation activity similar to wild-type in cultured cells (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
P387S missense gain of function MAP2K1 P387S does not lie within any known functional domains of the Map2k1 protein (UniProt.org). P387S confers a gain of function to the Map2k1 protein as demonstrated by constitutive phosphorylation of Mek and Erk (PMID: 22389471).
Q110_I111del deletion gain of function - predicted MAP2K1 Q110_I111del results in the deletion of two amino acids in the protein kinase domain of the Map2k1 protein from amino acids 110 to 111 (UniProt.org). Q110_I111del results in increased Erk phosphorylation in cultured cells (PMID: 32641410), and therefore, is predicted to lead to a gain of Map2k1 protein function.
Q164E missense no effect - predicted MAP2K1 Q164E lies within the protein kinase domain of the Map2k1 protein (UniProt.org). Q164E induces Erk phosphorylation similar to wild-type Map2k1 in cultured cells (PMID: 32641410), and therefore, is predicted to have no effect on Map2k1 protein function.
Q354H missense unknown MAP2K1 Q354H lies within the protein kinase domain of the Map2k1 protein (UniProt.org). Q354H has been identified in sequencing studies (PMID: 30111351), but has not been biochemically characterized and therefore, its effect on Map2k1 protein function is unknown (PubMed, Sep 2024).
Q56P missense gain of function MAP2K1 Q56P does not lie within any known functional domains of the Map2k1 protein (UniProt.org). Q56P confers a gain of function to the Map2k1 protein as indicated by increased kinase activity and the ability to transform cultured cells (PMID: 7651428, PMID: 22327936, PMID: 25351745) and increased proliferation in a competition assay (PMID: 36442478), and demonstrates resistance to some Mek and Braf inhibitors (PMID: 29753091). Y
Q56_G61delinsR indel gain of function - predicted MAP2K1 Q56_G61delinsR results in a deletion of six amino acids of the Map2k1 protein from amino acids 56 to 61, combined with the insertion of an arginine (R) at the same site (UniProt.org). Q56_G61delinsR results in proliferation under low serum conditions similar to wild-type Map2k1 in a competition assay, however, is predicted to lead to a gain of Map2k1 protein function as demonstrated by increased proliferation in a competition assay in normal serum and increased transformation activity in cultured cells, and confers resistance to Mek and Braf inhibitors in the context of BRAF V600E in culture (PMID: 36442478). Y
Q56_V60del deletion gain of function MAP2K1 Q56_V60del results in the deletion of five amino acids in the protein kinase domain of the Map2k1 protein from amino acids 56 to 60 (UniProt.org). Q56_V60del confers a gain of function to the Map2k1 protein as indicated by transformation capabilities and increased phosphorylation of Erk and S6k in culture (PMID: 26324360).
Q58* nonsense loss of function - predicted MAP2K1 Q58* results in a premature truncation of the Map2k1 protein at amino acid 58 of 393 (UniProt.org). Q58* results in proliferation similar to wild-type Map2k1 in a competition assay and transformation activity similar to wild-type in cultured cells (PMID: 36442478), however, due to the loss of the protein kinase domain (UniProt.org), is predicted to lead to a loss of Map2k1 protein function.
Q58H missense no effect - predicted MAP2K1 Q58H does not lie within any known functional domains of the Map2k1 protein (UniProt.org). Q58H induces Erk phosphorylation similar to wild-type Map2k1 in cultured cells (PMID: 32641410), and therefore, is predicted to have no effect on Map2k1 protein function.
Q58_E62del deletion gain of function MAP2K1 Q58_E62del results in the deletion of five amino acids in the Map2k1 protein from amino acis 58 to 62 (UniProt.org). Q58_E62del confers a gain of function to the Map2k1 protein as demonstrated by increased Mek and Erk phosphorylation relative to wild-type Map2k1 (PMID: 26566875, PMID: 25202140, PMID: 28935960).
R108Q missense no effect - predicted MAP2K1 R108Q lies within the protein kinase domain of the Map2k1 protein (UniProt.org). R108Q results in Erk phosphorylation (PMID: 32641410) and transformation activity similar to wild-type Map2k1 in culture (PMID: 36442478) and proliferation similar to wild-type in a competition assay (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
R108_N109del deletion gain of function - predicted MAP2K1 R108_N109del results in the deletion of two amino acids in the protein kinase domain of the Map2k1 protein from amino acids 108 to 109 (UniProt.org). R108_N109del results in increased Erk phosphorylation in cultured cells (PMID: 32641410), and therefore, is predicted to lead to a gain of Map2k1 protein function.
R113_E114del deletion no effect - predicted MAP2K1 R113_E114del results in the deletion of two amino acids in the protein kinase domain of the Map2k1 protein from amino acids 113 to 114 (UniProt.org). R113_E114del induces Erk phosphorylation similar to wild-type Map2k1 in cultured cells (PMID: 32641410), and therefore, is predicted to have no effect on Map2k1 protein function.
R201C missense no effect - predicted MAP2K1 R201C lies within the protein kinase domain of the Map2k1 protein (UniProt.org). R201C results in Erk phosphorylation (PMID: 32641410), proliferation in a competition assay, and transformation activity similar to wild-type in cultured cells (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
R201H missense no effect - predicted MAP2K1 R201H lies within the protein kinase domain of the Map2k1 protein (UniProt.org). R201H induces Erk phosphorylation similar to wild-type Map2k1 in cultured cells (PMID: 32641410), and therefore, is predicted to have no effect on Map2k1 protein function.
R227K missense no effect - predicted MAP2K1 R227K lies within the protein kinase domain of the Map2k1 protein (UniProt.org). R227K induces Erk phosphorylation similar to wild-type Map2k1 in cultured cells (PMID: 32641410), and therefore, is predicted to have no effect on Map2k1 protein function.
R260M missense unknown MAP2K1 R260M lies within the protein kinase domain of the Map2k1 protein (UniProt.org). R260M has not been characterized in the scientific literature and therefore, its effect on Map2k1 protein function is unknown (PubMed, Sep 2024).
R291K missense no effect - predicted MAP2K1 R291K lies within the protein kinase domain of the Map2k1 protein (UniProt.org). R291K results in proliferation similar to wild-type Map2k1 in a competition assay and transformation activity similar to wild-type in cultured cells (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
R349K missense no effect - predicted MAP2K1 R349K lies within the protein kinase domain of the Map2k1 protein (UniProt.org). R349K results in Erk phosphorylation (PMID: 32641410) and transformation activity similar to wild-type Map2k1 in cultured cells, and proliferation similar to wild-type in a competition assay (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
R47Q missense gain of function MAP2K1 R47Q lies within the negative regulatory region of the Map2k1 protein (PMID: 24241536). R47Q results in increased phosphorylation of Erk and is transforming in cell culture (PMID: 22327936).
R49C missense no effect - predicted MAP2K1 R49C lies within the negative regulatory region of the Map2k1 protein (PMID: 24241536). R49C results in proliferation similar to wild-type Map2k1 in a competition assay and transformation activity similar to wild-type in cultured cells (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
R49H missense no effect - predicted MAP2K1 R49H lies within the negative regulatory region of the Map2k1 protein (PMID: 24241536). R49H results in proliferation similar to wild-type Map2k1 in a competition assay and transformation activity (PMID: 36442478) and Erk phosphorylation similar to wild-type Map2k1 in cultured cells (PMID: 32641410) and therefore, is predicted to have no effect on Map2k1 protein function.
R49L missense gain of function MAP2K1 R49L lies within the negative regulatory region of the Map2k1 protein (PMID: 24241536). R49L confers a gain of function to the Map2k1 protein as demonstrated by transforming ability in culture and in vivo, and phosphorylation of Erk1/2 (PMID: 22327936).
R96K missense no effect - predicted MAP2K1 R96K lies within the protein kinase domain of the Map2k1 protein (UniProt.org). R96K results in Erk phosphorylation (PMID: 32641410) and transformation activity similar to wild-type Map2k1 in cultured cells (PMID: 36442478), and proliferation similar to wild-type in a competition assay (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
R96_K97del deletion no effect - predicted MAP2K1 R96_K97del results in the deletion of two amino acids in the protein kinase domain of the Map2k1 protein from amino acids 96 to 97 (UniProt.org). R96_K97del induces Erk phosphorylation similar to wild-type Map2k1 in cultured cells (PMID: 32641410), and therefore, is predicted to have no effect on Map2k1 protein function.
S123P missense unknown MAP2K1 S123P lies within the protein kinase domain of the Map2k1 protein (UniProt.org). S123P has been identified in the scientific literature (PMID: 26566875), but has not been biochemically characterized and therefore, its effect on Map2k1 protein function is unknown (PubMed, Sep 2024).
S123T missense gain of function MAP2K1 S123T lies within the protein kinase domain of the Map2k1 protein (UniProt.org). S123T confers a gain of function to the Map2k1 protein as demonstrated by increased Mek and Erk phosphorylation relative to wild-type Map2k1 (PMID: 26566875).
S150F missense no effect - predicted MAP2K1 S150F lies within the protein kinase domain of the Map2k1 protein (UniProt.org). S150F results in proliferation similar to wild-type Map2k1 in a competition assay and transformation activity similar to wild-type in cultured cells (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
S200Y missense no effect - predicted MAP2K1 S200Y lies within the protein kinase domain of the Map2k1 protein (UniProt.org). S200Y results in proliferation similar to wild-type Map2k1 in a competition assay and transformation activity similar to wild-type in cultured cells (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
S212N missense no effect - predicted MAP2K1 S212N lies within the protein kinase domain of the Map2k1 protein (UniProt.org). S212N results in proliferation similar to wild-type Map2k1 in a competition assay and transformation activity similar to wild-type in cultured cells (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
S218D missense gain of function MAP2K1 S218D lies within the protein kinase domain of the Map2k1 protein (UniProt.org). S218D confers a gain of function to the Map2k1 protein as indicated by increased Map2k1 activity in the absence of growth factor, cell growth in culture, and tumor formation in mouse models (PMID: 7936666).
S222A missense no effect - predicted MAP2K1 S222A lies within the protein kinase domain of the Map2k1 protein (UniProt.org). S222A results in similar cell proliferation and viability levels as wild-type Map2k1 (PMID: 29533785), and therefore, is predicted to have no effect on Map2k1 protein function.
S222D missense unknown MAP2K1 S222D lies within the protein kinase domain of the Map2k1 protein (UniProt.org). S222D results in constitutive activation of Map2k1 when combined with S218D or S226D in culture (PMID: 7936666, PMID: 12506122), but has not been individually characterized and therefore, its effect on Map2k1 protein function is unknown.
S231L missense no effect - predicted MAP2K1 S231L lies within the protein kinase domain of the Map2k1 protein (UniProt.org). S231L results in proliferation similar to wild-type Map2k1 in a competition assay and transformation activity similar to wild-type in cultured cells (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
S241Y missense gain of function - predicted MAP2K1 S241Y lies within the protein kinase domain of the Map2k1 protein (UniProt.org). S241Y results in transformation activity and proliferation in a competition assay in normal serum conditions similar to wild-type Map2k1 but increased proliferation in a competition assay in low serum conditions (PMID: 36442478), and therefore, is predicted to lead to a gain of Map2k1 protein function.
S327T missense no effect - predicted MAP2K1 S327T lies within the protein kinase domain of the Map2k1 protein (UniProt.org). S327T results in Erk phosphorylation (PMID: 32641410) and transformation activity similar to wild-type Map2k1 in cultured cells and proliferation similar to wild-type in a competition assay (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
S331R missense no effect - predicted MAP2K1 S331R lies within the protein kinase domain of the Map2k1 protein (UniProt.org). S331R results in proliferation similar to wild-type Map2k1 in a competition assay and transformation activity similar to wild-type in cultured cells (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
S72G missense no effect - predicted MAP2K1 S72G lies within the protein kinase domain of the Map2k1 protein (UniProt.org). S72G results in proliferation similar to wild-type Map2k1 in a competition assay and transformation activity similar to wild-type in cultured cells (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
S86A missense no effect - predicted MAP2K1 S86A lies within the protein kinase domain of the Map2k1 protein (UniProt.org). S86A results in Erk phosphorylation similar to wild-type Map2k1 in cultured cells (PMID: 32641410), and transformation activity and proliferation similar to wild-type Map2k1 in cultured cells and a competition assay, respectively (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
T28I missense gain of function - predicted MAP2K1 T28I lies within the Erk binding domain of the Map2k1 protein (PMID: 26566875). T28I is predicted to confer a gain of function to the Map2k1 protein as demonstrated by increased Mek phosphorylation in culture (PMID: 26566875).
T55P missense unknown MAP2K1 T55P does not lie within any known functional domains of the Map2k1 protein (UniProt.org). T55P results in autophosphorylation levels similar to wild-type Map2k1 in an in vitro assay (PMID: 29753091), but leads to increased Erk phosphorylation in cultured cells in another study (PMID: 32641410), and therefore, its effect on Map2k1 protein function is unknown.
V127M missense no effect - predicted MAP2K1 V127M lies within the protein kinase domain of the Map2k1 protein (UniProt.org). V127M results in proliferation similar to wild-type Map2k1 in a competition assay and transformation activity similar to wild-type in cultured cells (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
V154I missense no effect - predicted MAP2K1 V154I lies within the protein kinase domain of the Map2k1 protein (UniProt.org). V154I results in proliferation similar to wild-type Map2k1 in a competition assay and transformation activity similar to wild-type in cultured cells (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
V211A missense unknown MAP2K1 V211A lies within the protein kinase domain of the Map2k1 protein (UniProt.org). V211A has not been characterized in the scientific literature and therefore, its effect on Map2k1 protein function is unknown (PubMed, Sep 2024).
V211D missense gain of function MAP2K1 V211D lies within the protein kinase domain of the Map2k1 protein (UniProt.org). V211D confers a gain of function to Map2k1, as demonstrated by increased phosphorylation of Mek and Erk in cultured cells and in vitro kinase assays, and also demonstrates Braf and Mek inhibitor resistance (PMID: 29753091, PMID: 19915144, PMID: 31227518). Y
V224M missense no effect - predicted MAP2K1 V224M lies within the protein kinase domain of the Map2k1 protein (UniProt.org). V224M results in proliferation similar to wild-type Map2k1 in a competition assay and transformation activity similar to wild-type in cultured cells (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
V258I missense no effect - predicted MAP2K1 V258I lies within the protein kinase domain of the Map2k1 protein (UniProt.org). V258I induces Erk phosphorylation similar to wild-type Map2k1 in cultured cells (PMID: 32641410), and therefore, is predicted to have no effect on Map2k1 protein function.
V60E missense gain of function MAP2K1 V60E does not lie within any known functional domains of the Map2k1 protein (UniProt.org). V60E confers a gain of function to Map2k1 as demonstrated by increased Erk signaling (PMID: 28263969), transformation activity in cultured cells and increased proliferation in a competition assay (PMID: 36442478), and tumor formation in orthotopic mouse models (PMID: 28263969), and demonstrates resistance to some Braf inhibitors (PMID: 24265153, PMID: 28263969, PMID: 36442478). Y
V60M missense gain of function MAP2K1 V60M does not lie within any known functional domains of the Map2k1 protein (UniProt.org). V60M confers a gain of function to the Map2k1 protein as demonstrated by increased Mek and Erk phosphorylation relative to wild-type Map2k1 (PMID: 26566875).
V93F missense no effect - predicted MAP2K1 V93F lies within the protein kinase domain of the Map2k1 protein (UniProt.org). V93F results in proliferation similar to wild-type Map2k1 in a competition assay and transformation activity similar to wild-type in cultured cells (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.
W247* nonsense unknown MAP2K1 W247* results in a premature truncation of the Map2k1 protein at amino acid 247 of 393 (UniProt.org). W247* demonstrates slower growth than wild-type in low nutrient cell culture (PMID: 36442478), but has not been fully biochemically characterized and therefore, its effect on Map2k1 protein function is unknown.
wild-type none no effect Wild-type MAP2K1 indicates that no mutation has been detected within the MAP2K1 gene.
Y130C missense unknown MAP2K1 Y130C lies within the protein kinase domain of the Map2k1 protein (UniProt.org). Y130C demonstrates a phosphorylation profile similar to wild-type Map2k1 (PMID: 29753091), but in another study results in increased Erk phosphorylation in cell culture (PMID: 16439621, PMID: 18060073, PMID: 17981815, PMID: 31972311), and elevated cell cycle progression and colony formation compared to wild-type Map2k1 (PMID: 31972311), and therefore, its effect on Map2k1 protein function is unknown.
Y130H missense unknown MAP2K1 Y130H lies within the protein kinase domain of the Map2k1 protein (UniProt.org). Y130H results in autophosphorylation levels similar to wild-type Map2k1 in an in vitro assay (PMID: 29753091), but leads to increased Erk phosphorylation in cultured cells in another study (PMID: 32641410), and therefore, its effect on Map2k1 protein function is unknown.
Y130N missense no effect - predicted MAP2K1 Y130N lies within the protein kinase domain of the Map2k1 protein (UniProt.org). Y130N has been demonstrated to result in autophosphorylation levels similar to wild-type Map2k1 in an in vitro assay (PMID: 29753091), and therefore, is predicted to have no effect on Map2k1 protein function.
Y134C missense gain of function - predicted MAP2K1 Y134C lies within the protein kinase domain of the Map2k1 protein (UniProt.org). Y134C results in proliferation similar to wild-type Map2k1 in a competition assay and transformation activity similar to wild-type Map2k1 (PMID: 36442478) but increased Erk phosphorylation in cultured cells (PMID: 32641410), and therefore, is predicted to lead to a gain of Map2k1 protein function.
Y229H missense no effect - predicted MAP2K1 Y229H lies within the protein kinase domain of the Map2k1 protein (UniProt.org). Y229H results in proliferation similar to wild-type Map2k1 in a competition assay and transformation activity similar to wild-type in cultured cells (PMID: 36442478), and therefore, is predicted to have no effect on Map2k1 protein function.