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Gene | BRAF |
Variant | V600M |
Impact List | missense |
Protein Effect | gain of function - predicted |
Gene Variant Descriptions | BRAF V600M (previously reported as V599) lies within the activation segment of the kinase domain of the Braf protein (PMID: 15035987). V600M results in intermediate Braf kinase activity in cell culture (PMID: 28783719), and in one of two cell lines increased cell proliferation and cell viability compared to wild-type Braf (PMID: 29533785), and therefore, is predicted to lead to a gain of Braf protein function. |
Associated Drug Resistance | |
Category Variants Paths |
BRAF mutant BRAF act mut BRAF V600M BRAF mutant BRAF V600X BRAF V600M |
Transcript | NM_004333.6 |
gDNA | chr7:g.140753337C>T |
cDNA | c.1798G>A |
Protein | p.V600M |
Source Database | RefSeq |
Genome Build | GRCh38/hg38 |
Transcript | gDNA | cDNA | Protein | Source Database | Genome Build |
---|---|---|---|---|---|
NM_004333 | chr7:g.140753337C>T | c.1798G>A | p.V600M | RefSeq | GRCh38/hg38 |
NM_001378468.1 | chr7:g.140753337C>T | c.1798G>A | p.V600M | RefSeq | GRCh38/hg38 |
NM_004333.6 | chr7:g.140753337C>T | c.1798G>A | p.V600M | RefSeq | GRCh38/hg38 |
NM_004333.5 | chr7:g.140753337C>T | c.1798G>A | p.V600M | RefSeq | GRCh38/hg38 |
NM_001354609.2 | chr7:g.140753337C>T | c.1798G>A | p.V600M | RefSeq | GRCh38/hg38 |
NM_001354609.1 | chr7:g.140753337C>T | c.1798G>A | p.V600M | RefSeq | GRCh38/hg38 |
NM_001378474.1 | chr7:g.140753337C>T | c.1798G>A | p.V600M | RefSeq | GRCh38/hg38 |
XM_005250045 | chr7:g.140753337C>T | c.1798G>A | p.V600M | RefSeq | GRCh38/hg38 |
Molecular Profile | Indication/Tumor Type | Response Type | Therapy Name | Approval Status | Evidence Type | Efficacy Evidence | References |
---|---|---|---|---|---|---|---|
BRAF V600M | lung non-small cell carcinoma | predicted - sensitive | Vemurafenib | Case Reports/Case Series | Actionable | In a Phase II trial, Zelboraf (vemurafenib) treatment resulted in a objective response rate of 44.8% (43/96), median duration of response of 6.4 months, median progression-free survival (PFS) of 5.2 months, and median overall survival of 10 months in non-small cell lung cancer patients with BRAF V600 mutations, with a PFS of 5.9 months in the patient harboring BRAF V600M (PMID: 31959346; NCT02304809). | 31959346 |