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Gene | BRAF |
Variant | T599dup |
Impact List | duplication |
Protein Effect | gain of function |
Gene Variant Descriptions | BRAF T599dup (also referred to T599_V600insT) indicates the insertion of the duplicate amino acid, threonine (T)-599, in the protein kinase domain of the Braf protein (UniProt.org). T599dup results in increased kinase activity (PMID: 21190184), increased phosphorylation of Mek and Erk, and transformation in cell culture (PMID: 21190184, PMID: 38662982). |
Associated Drug Resistance | |
Category Variants Paths |
BRAF mutant BRAF act mut BRAF T599dup |
Transcript | NM_004333.6 |
gDNA | chr7:g.140753338_140753340dupTGT |
cDNA | c.1795_1797dupACA |
Protein | p.T599dup |
Source Database | RefSeq |
Genome Build | GRCh38/hg38 |
Transcript | gDNA | cDNA | Protein | Source Database | Genome Build |
---|---|---|---|---|---|
NM_001378472.1 | chr7:g.140749326_140749328 | c.1795_1797 | p.T599 | RefSeq | GRCh38/hg38 |
NM_001354609.2 | chr7:g.140753338_140753340 | c.1795_1797 | p.T599 | RefSeq | GRCh38/hg38 |
XM_005250045 | chr7:g.140753338_140753340dupTGT | c.1795_1797dupACA | p.T599dup | RefSeq | GRCh38/hg38 |
NM_001378468.1 | chr7:g.140753338_140753340 | c.1795_1797 | p.T599 | RefSeq | GRCh38/hg38 |
NM_004333 | chr7:g.140753338_140753340dupTGT | c.1795_1797dupACA | p.T599dup | RefSeq | GRCh38/hg38 |
NM_001354609.1 | chr7:g.140753338_140753340 | c.1795_1797 | p.T599 | RefSeq | GRCh38/hg38 |
NM_004333.5 | chr7:g.140753338_140753340dupTGT | c.1795_1797dupACA | p.T599dup | RefSeq | GRCh38/hg38 |
NM_004333.6 | chr7:g.140753338_140753340dupTGT | c.1795_1797dupACA | p.T599dup | RefSeq | GRCh38/hg38 |
NM_001378473.1 | chr7:g.140749326_140749328 | c.1795_1797 | p.T599 | RefSeq | GRCh38/hg38 |
NM_001378474.1 | chr7:g.140753338_140753340 | c.1795_1797 | p.T599 | RefSeq | GRCh38/hg38 |
Molecular Profile | Indication/Tumor Type | Response Type | Therapy Name | Approval Status | Evidence Type | Efficacy Evidence | References |
---|---|---|---|---|---|---|---|
BRAF T599dup | Advanced Solid Tumor | sensitive | Trametinib | Preclinical - Cell culture | Actionable | In a preclinical study, Mekinist (trametinib) inhibited downstream signaling and viability in transformed cells expressing BRAF T599dup in culture (PMID: 38662982). | 38662982 |
BRAF T599dup | Advanced Solid Tumor | sensitive | Dabrafenib | Preclinical - Cell culture | Actionable | In a preclinical study, Tafinlar (dabrafenib) inhibited downstream signaling and viability in transformed cells expressing BRAF T599dup in culture (PMID: 38662982). | 38662982 |
BRAF T599dup | melanoma | predicted - resistant | Vemurafenib | Case Reports/Case Series | Actionable | In a clinical case study, Zelboraf (vemurafenib) resulted in disease progression in two patients with metastatic melanoma harboring BRAF T599dup (PMID: 35319964). | 35319964 |
BRAF T599dup | colorectal cancer | resistant | Cetuximab | Preclinical - Pdx | Actionable | In preclinical study, a colorectal cancer patient-derived xenograft (PDX) model harboring BRAF T599dup was resistant to Erbitux (cetuximab) treatment (PMID: 31515458). | 31515458 |
BRAF T599dup | melanoma | predicted - sensitive | Dabrafenib + Trametinib | Case Reports/Case Series | Actionable | In a clinical case study, Tafinlar (dabrafenib) and Mekinist (trametinib) combination resulted in partial metabolic remission within six weeks in a patient with metastatic melanoma harboring BRAF T599dup (PMID: 29494756). | 29494756 |
BRAF T599dup | melanoma | predicted - sensitive | Dabrafenib + Trametinib | Case Reports/Case Series | Actionable | In a clinical study, a combination of Tafinlar (dabrafenib) and Mekinist (trametinib) resulted in partial responses in 4 patients with metastatic melanoma harboring BRAF T599dup, with one patient also harboring CTNNB1 I35T (PMID: 35319964). | 35319964 |
BRAF T599dup | lung adenocarcinoma | predicted - sensitive | Dabrafenib + Trametinib | Case Reports/Case Series | Actionable | In a clinical case study, a patient with lung adenocarcinoma harboring BRAF T599dup demonstrated a response to the combination of Tafinlar (dabrafenib) and Mekinist (trametinib), with regression of the left lower lobe bronchial tumor (PMID: 33704186). | 33704186 |
BRAF T599dup | lung adenocarcinoma | predicted - sensitive | Dabrafenib + Trametinib | Case Reports/Case Series | Actionable | In a clinical case study, treatment with the combination of Tafinlar (dabrafenib) and Mekinist (trametinib) resulted in 12.3% tumor shrinkage in a patient with lung adenocarcinoma harboring BRAF T599dup along with KRAS Q61H, however, treatment was discontinued for adverse events (PMID: 38662982). | 38662982 |
BRAF T599dup | ganglioglioma | predicted - sensitive | Dabrafenib + Trametinib | Case Reports/Case Series | Actionable | In a clinical case study, the combination of Tafinlar (dabrafenib) and Mekinist (trametinib) resulted in clinical improvement and tumor shrinkage with ongoing stable disease for at least 15 months in a pediatric patient with ganglioglioma harboring BRAF T599dup (PMID: 33637608). | 33637608 |
BRAF T599dup | melanoma | predicted - sensitive | Binimetinib + Encorafenib | Case Reports/Case Series | Actionable | In a clinical case study, a combination of Mektovi (binimetinib) and Braftovi (encorafenib) resulted in regression of lymphadenopathy, lung nodules, and subcutaneous nodules in a patient with metastatic melanoma harboring BRAF T599dup, and at 9 months from treatment initiation, a partial response was still observed (PMID: 35319964). | 35319964 |
BRAF T599dup | colorectal cancer | predicted - resistant | Fluorouracil + Leucovorin + Oxaliplatin + Panitumumab | Case Reports/Case Series | Actionable | In a clinical study, the combination of Vectibix (panitumumab) with FOLFOX as a first-line therapy resulted in progressive disease in a patient with metastatic colorectal cancer harboring BRAF T599dup (PMID: 31515458). | 31515458 |